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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: May 4, 2026

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

A high-throughput method for GMO multi-detection using a microfluidic dynamic array.

Fábio Cristiano Angonesi Brod¹, Jeroen P van Dijk, Marleen M Voorhuijzen

¹RIKILT Wageningen UR, P.O. Box 230, 6700 AE, Wageningen, The Netherlands.

Analytical and Bioanalytical Chemistry

|December 21, 2013

Summary

This study introduces a microfluidic dynamic array for high-throughput detection of genetically modified organisms (GMOs). This innovative DNA-based method offers a cost-effective solution for GMO testing and labeling requirements.

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Last Updated: May 4, 2026

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Area of Science:

Agricultural Biotechnology
Molecular Biology
Analytical Chemistry

Background:

Increasing global production of genetically modified crops necessitates efficient GMO detection methods.
Standard real-time PCR methods for GMO analysis can become prohibitively expensive with a growing number of detectable GMO events.
There is a need for high-throughput, multi-detection systems for GMO analysis to meet regulatory demands.

Purpose of the Study:

To evaluate a microfluidic dynamic array as a high-throughput system for DNA-based GMO detection.
To assess the suitability of the Fluidigm system for analyzing multiple GMOs and species-specific reference genes simultaneously.
To determine the limit of detection for low-level GMO presence using this microfluidic platform.

Main Methods:

Preparation and preamplification of six test samples with varying complexity.
Analysis of samples using a microfluidic dynamic array (Fluidigm system).
Utilized twenty-eight distinct assays targeting various DNA elements, specific genetically modified (GM) events, and species-specific reference genes.

Main Results:

The microfluidic dynamic array system demonstrated high performance, with the majority of assays yielding expected results.
The system successfully detected target elements present at concentrations as low as 0.06%.
The Fluidigm system proved effective for multi-detection of GMOs and reference genes.

Conclusions:

The microfluidic dynamic array system is a promising and suitable platform for high-throughput, multi-detection of GMOs.
This approach offers a potentially more cost-effective solution compared to standard methods for complex GMO testing scenarios.
The technology supports accurate GMO identification and quantification, aiding in regulatory compliance for food labeling.