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Updated: May 4, 2026

Subnanometer-resolution Structural Determination of Hemagglutinin from Cryo-electron Tomography of Influenza Viruses
Published on: November 7, 2025
Location and architecture of an antibody-binding site of influenza A virus nucleoprotein
Natalia L Varich1, Galina K Sadykova, Alexei G Prilipov
1D. I. Ivanovsky Institute of Virology, Gamaleya Str. 16, 123098, Moscow, Russia, nataliavarich@rambler.ru.
Abstract:
Amino acid positions recognized by monoclonal antibodies (MAbs) in the influenza A virus nucleoprotein (NP) have been reported. As these residues were scattered in the three-dimensional (3D) structure of NP, no patterns of the architecture of antibody-binding sites could be inferred. Here, we used site-specific mutagenesis and ELISA to screen the amino acids surrounding position 470 recognized by the MAb 3/1 as a linear epitope. Ten amino acid residues involved in the reaction of NP with the MAb 3/1 and the MAb 469/6 were identified. Our data are the first to outline a compact site recognized by MAbs in the 3D structure of the influenza virus NP.
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