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Updated: May 4, 2026

Simple In-House Ultra-High Performance Capillary Column Manufacturing with the FlashPack Approach
Published on: December 4, 2021
Porous layer open tubular columns in capillary liquid chromatography
David A Collins1, Ekaterina P Nesterenko, Brett Paull
1Irish Separation Science Cluster, Dublin City University, Glasnevin, Dublin 9, Ireland. ekaterina.nesterenko@dcu.ie.
Abstract:
This review covers the latest developments and applications of porous layer open tubular columns in capillary liquid chromatography. Here, the authors provide a concise background on the fundamentals of porous layer open tubular columns, their fabrication and application. Over the past two decades, growing interest in the areas of proteomics and hyphenated LC-MS techniques have played a large part in the development of porous layer structures within capillary formats due to their high permeability, excellent efficiency and exceptional peak capacity. This review gives a brief overview of open tubular columns in general, however, it focuses predominantly on the applications of covalently bonded porous layer open tubular columns in liquid chromatography. Open tubular columns containing non-bonded or electrostatically attached layers will not be discussed in detail.
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