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Updated: May 4, 2026
A Multi-detection Assay for Malaria Transmitting Mosquitoes
Published on: February 28, 2015
[Analysis of K76T Pfcrt genetic polymorphism in imported Plasmodium falciparum]
Shui-mao Zhou1, Yan Yang, Zhi Che
1Wuhan Centre for Disease Control and Prevention, Wuhan 430015, China.
Objective:
To identify the incidence of the K76T mutation in Pfcrt genes of imported Plasmodium falciparum.
Methods:
The blood samples were collected from returnees infected with P. falciparum in endemic areas of Southeast Asia and Africa from 2008 to 2012. According to the Pfcrt gene sequence of P. falciparum, nested PCR primers were designed. Nested PCR-RFLP was applied with falciparum DNA in the blood samples as templates.
Results:
Among 92 blood samples of P. falciparum, the mutant Pfcrt alleles were found in 50 samples (54.3%), and the wild type Pfcrt alleles were found in 42 samples (45.7%). There were 33 samples (47.1%) with mutant Pfcrt alleles and 37 samples (52.9%) with wild type, respectively, from Africa. There were 17 samples (77.3%) with mutant Pfcrt alleles and 5 samples (22.7%) with wild type, respectively, from Southeast Asia. There was a significant difference between the Africa group and the Southeast Asia group (Chi(2) = 6.12, P < 0.05).
Conclusion:
The incidence of Pfcrt gene mutation is different among P. falciparum isolates from different regions. Therefore, Pfcrt K76T has an application value in the surveillance of the imported falciparum chloroquine-resistance.
Insights
The K76T mutation in the Pfcrt gene of Plasmodium falciparum shows varying incidence in different regions. This mutation is valuable for monitoring imported falciparum chloroquine resistance.
Area of Science:
- Molecular parasitology
- Genetics of infectious diseases
- Drug resistance mechanisms
Context:
- Plasmodium falciparum is a significant cause of malaria.
- Chloroquine resistance in P. falciparum is a global health concern.
- The Pfcrt gene is a key determinant of chloroquine resistance.
Purpose:
- To determine the incidence of the K76T mutation in Pfcrt genes of imported Plasmodium falciparum.
- To compare the mutation rates between isolates from Africa and Southeast Asia.
Summary:
- A study analyzed 92 blood samples of P. falciparum from travelers returning from endemic areas.
- Nested PCR-RFLP detected Pfcrt K76T mutations, finding mutant alleles in 54.3% of samples.
- Incidence was higher in Southeast Asia (77.3% mutant) compared to Africa (47.1% mutant), with a significant regional difference.
Impact:
- The Pfcrt K76T mutation's varying incidence highlights regional differences in chloroquine resistance.
- This finding supports the use of Pfcrt K76T as a marker for surveillance of imported falciparum chloroquine resistance.
- Understanding mutation prevalence aids in developing effective malaria treatment and control strategies.
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