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[Comparison of ELISA and pharmacia UNICAP detection reagent for house dust mite specific IgG4]
Genyu Luo1, Rui Gong2, Zhenkui Gong1
1Jiangsu Huaguan Biotechnology Joint Stock Company Ltd, Taizhou, 225300, China.
Objective:
To compare the ELISA and Pharmica unicap reagents of Dermatophagoides pteronyssinus specific IgG4 in clinic.
Method:
The stability of ELISA reagent was studied first with parameters of intra and inter assay variations (%CV). Calibration curve of the two reagents was compared. Sixty-five serum samples before or after standardized specific immunotherapy were examined with the two methods, correlation coefficient was calculated.
Result:
The stability of ELISA reagent was good with low intra and inter assay variations (3.48% and 5.78%). Calibration curve of the two reagents was similar. The correlation coefficient was 0.93 between the two test methods.
Conclusion:
The results of ELISA and Pharmica unicap reagents of Dermatophagoides pteronyssinus specific IgG4 were highly consistent. The ELISA reagents could be applied in clinic.
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