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Updated: May 4, 2026

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays
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Microarray technologies for intracellular kinome analysis.

T Yamamoto, T Mori, Y Katayama1

  • 1Department of Applied Chemistry, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819- 0395, Japan. ykatatcm@mail.cstm.kyushu-u.ac.jp.

Current Medicinal Chemistry
|December 24, 2013
PubMed
Summary
This summary is machine-generated.

Microarray-based kinomics offer a simple, high-throughput alternative to mass spectrometry for studying protein kinases. While less quantitative, they are valuable for drug development and disease diagnostics.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Proteomics

Background:

  • Intracellular protein kinases play crucial roles in cellular signaling pathways.
  • Conventional mass spectrometry-based kinomics provide quantitative analysis but can be complex and time-consuming.
  • Microarray-based kinomics have emerged as complementary tools for studying protein kinase activity and presence.

Purpose of the Study:

  • To review the advantages, recent advancements, and limitations of various microarray-based kinomics approaches.
  • To compare microarray-based kinomics with conventional mass spectrometry-based methods.
  • To highlight the practical applications of microarray-based kinomics in drug development and disease diagnostics.

Main Methods:

  • Review of substrate peptide and protein microarrays.
  • Review of anti-protein kinase antibody microarrays.
  • Review of reverse protein microarrays.

Main Results:

  • Microarray-based kinomics offer simplicity and high throughput compared to mass spectrometry.
  • These methods are less adept at quantitative kinomics evaluation.
  • Representative microarray types include substrate peptide/protein, anti-protein kinase antibody, and reverse protein microarrays.

Conclusions:

  • Microarray-based kinomics are unique tools for practical analyses due to their simplicity and high throughput.
  • They are well-suited for monitoring drug effects on cellular kinomics in drug development.
  • These methods can aid in the diagnosis and prognosis of diseases based on kinomic profiles.