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DNAJB1 stabilizes MDM2 and contributes to cancer cell proliferation in a p53-dependent manner

Min Qi1, Jianglin Zhang2, Weiqi Zeng2

  • 1Department of Plastic Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, PR China.

Biochimica Et Biophysica Acta
|December 24, 2013
PubMed

Insights

DNAJB1 stabilizes MDM2, inhibiting p53 degradation and activating the p53 pathway. This DNAJB1 function suppresses tumor growth in a p53-dependent manner, highlighting its role in cancer.

Area of Science:

  • Molecular Biology
  • Cancer Biology
  • Protein Interactions

Background:

  • MDM2 and MDMX are key regulators of the tumor suppressor p53.
  • Their distinct roles in p53 regulation require further elucidation.

Purpose of the Study:

  • To identify novel proteins interacting with MDM2.
  • To characterize the functional relationship between DNAJB1 and MDM2 in the context of p53 regulation.

Main Methods:

  • Yeast two-hybrid (Y2H) screening using the MDM2 acidic domain.
  • In vitro and in vivo binding assays.
  • Western blotting to assess protein stability and ubiquitination.
  • Cellular assays to evaluate pathway activity and cell growth.
  • RNA interference (siRNA) and viral protein expression (HPV E6).

Main Results:

  • DNAJB1 specifically binds to MDM2, not MDMX.
  • DNAJB1 enhances MDM2 stability at the post-translational level.
  • DNAJB1 inhibits MDM2-mediated ubiquitination and degradation of p53.
  • Depletion of DNAJB1 activates the Rb/E2F pathway and promotes cancer cell growth, dependent on p53.
  • HPV E6 or p53 siRNA abrogates the effects of DNAJB1 depletion.

Conclusions:

  • DNAJB1 is a novel MDM2-interacting protein.
  • DNAJB1 functions as a p53-dependent tumor suppressor by stabilizing MDM2 and promoting p53 activity.
  • Targeting DNAJB1 may offer therapeutic strategies in cancer treatment.
Keywords:
DNAJB1HSP70MDM2p21p53

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