Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Membrane Asymmetry Regulating Transporters01:19

Membrane Asymmetry Regulating Transporters

6.0K
Enzymes like flippase, floppase, and scramblase transfer phospholipids from one layer to another in the membrane, thereby affecting membrane asymmetry.
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
6.0K
Cholesterol: Significance and Regulation01:29

Cholesterol: Significance and Regulation

2.1K
Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
Considering cholesterol and...
2.1K
Carrier-Mediated Transport01:06

Carrier-Mediated Transport

1.6K
Carrier-mediated transport is a pivotal process in drug absorption, particularly for lipid-insoluble drugs, and encompasses facilitated diffusion and active transport. Facilitated diffusion allows drugs to move along their concentration gradient without energy expenditure, while active transport utilizes ATP to drive drug movement against this gradient.
Active transport involves two types of membrane-spanning transporters: uptake and efflux. Uptake transporters are expressed in the small...
1.6K
Lipid Catabolism01:25

Lipid Catabolism

1.4K
Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...
1.4K
Hepatic Drug Clearance: Role of Transporters01:14

Hepatic Drug Clearance: Role of Transporters

388
In the liver and bile canaliculi, influx and efflux transporters modification can influence intrinsic clearance. Transporters play a significant role in moving drugs within liver cells. Elaborate models, such as the Biopharmaceutical Classification System (BCS), are essential to relate transporters to drug disposition. This system categorizes drugs into four classes based on solubility and permeability, providing insights into elimination routes and the effects of transporters following oral...
388
Lipid Absorption01:24

Lipid Absorption

3.9K
Dietary triglycerides from chyme in the duodenum are mixed with bile salts produced by the liver to emulsify fats. As a result, large droplets are broken down into smaller ones, increasing the surface area for enzymatic action. Once emulsified, pancreatic lipases hydrolyze the triglycerides into free fatty acids and monoglycerides.
These breakdown products bind with bile salts and lecithin to form micelles, which quickly pass between microvilli to come in close contact with the apical...
3.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pressure points: A pilot study using the NASA-TLX tool to measure the intensity of Acute care surgery work.

American journal of surgery·2026
Same author

A review of multi-omics integration techniques across five machine learning method families.

Bioinformatics advances·2026
Same author

Muscle mass, function and performance across the adult lifespan: the study of muscle, mobility and ageing.

Age and ageing·2026
Same author

Extreme Size and Irradiance Dependence in High-Resolution Vat Photopolymerization of Hydrogels.

Small methods·2026
Same author

Factors associated with protection from MASLD in type 2 diabetes: A prospective study integrating longitudinal MRI/MRE and stable isotope tracing.

JHEP reports : innovation in hepatology·2026
Same author

Artificial intelligence, machine learning-based automated fibrosis quantification in preclinical models of pulmonary fibrosis.

Respiratory research·2026

Related Experiment Video

Updated: May 4, 2026

Cholesterol Efflux Assay
07:54

Cholesterol Efflux Assay

Published on: March 6, 2012

29.4K

Reverse cholesterol transport fluxes.

Marc Hellerstein1, Scott Turner

  • 1aKineMed, Inc., Emeryville bDepartment of Nutritional Sciences and Toxicology, University of California, Berkeley cDepartment of Medicine, Division of Endocrinology and Metabolism, University of California, San Francisco, California, USA.

Current Opinion in Lipidology
|December 24, 2013
PubMed
Summary
This summary is machine-generated.

Recent studies reveal that reverse cholesterol transport (RCT) steps are independent and weakly linked to HDL cholesterol. This suggests a revised view of RCT, moving beyond a solely HDL-centric perspective for atherosclerosis understanding.

More Related Videos

High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay
08:18

High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay

Published on: January 7, 2019

8.6K
High-Density Lipoprotein-Specific Phospholipid Efflux Assay
07:08

High-Density Lipoprotein-Specific Phospholipid Efflux Assay

Published on: September 30, 2025

564

Related Experiment Videos

Last Updated: May 4, 2026

Cholesterol Efflux Assay
07:54

Cholesterol Efflux Assay

Published on: March 6, 2012

29.4K
High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay
08:18

High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay

Published on: January 7, 2019

8.6K
High-Density Lipoprotein-Specific Phospholipid Efflux Assay
07:08

High-Density Lipoprotein-Specific Phospholipid Efflux Assay

Published on: September 30, 2025

564

Area of Science:

  • Cardiovascular Biology
  • Lipid Metabolism
  • Atherosclerosis Research

Background:

  • High-density lipoprotein (HDL) plays a key role in atheroprotection via reverse cholesterol transport (RCT).
  • Quantifying cholesterol flux through the RCT pathway has been challenging until recently.
  • Understanding HDL function and RCT's role in atherosclerosis requires improved quantification methods.

Purpose of the Study:

  • To review new information on cholesterol flux through the RCT pathway.
  • To enhance understanding of HDL function and RCT's role in atherosclerosis.
  • To highlight recent quantitative insights into RCT.

Main Methods:

  • Review of recent clinical and nonclinical studies on cholesterol flux.
  • Analysis of quantitative features of in vivo cholesterol fluxes.
  • Examination of mechanistic data on RCT components.

Main Results:

  • Individual RCT steps are independent and show weak correlation with plasma HDL cholesterol levels.
  • Nonclinical studies highlight the role of apoB particles, serum albumin, and erythrocytes in free cholesterol flux.
  • Findings suggest a potential need to revise the HDL-centric view of RCT.

Conclusions:

  • The complexity of the RCT pathway involves an increasing number of lipoproteins and other factors.
  • Further human research is crucial to identify key RCT pathway components relevant to atherosclerosis pathophysiology and treatment.
  • Understanding specific steps within RCT is vital for developing new therapeutic strategies.