The dehydrin wzy2 promoter from wheat defines its contribution to stress tolerance

Dehydrins (DHNs), which are stress-related proteins, are important for plant survival under various abiotic and biotic stresses. To elucidate the regulatory mechanisms of wheat-derived DHNs under these stresses, we characterized the DHN wzy2 promoter of the wheat cultivar Zhengyin 1 and studied its contribution to stress tolerance. Sequence analysis indicated that the wzy2 gene contains one 109-bp intron inserted in the nucleotide sequence encoding the S-motif and characterized by a GT-AG border. The wzy2 promoter was revealed to contain several potential stress-related cis-acting regulatory elements, including elements responsive to abscisic acid (ABA; ABREs), anoxia (GC motifs), low temperature (LTREs), auxin (TGA elements), methyl jasmonate (MeJA; TGACG motifs), and gibberellin (TATC boxes). Quantitative real-time PCR analysis showed that transcript accumulation occurred in response to low temperature, anoxia, indoleacetic acid, MeJA, ABA, and gibberellin (GA) treatments. Histochemical analysis of GUS expression demonstrated that wzy2 promoter activity could be upregulated by low temperature, anoxia, ABA, and GA treatments. Interestingly, wzy2 promoter element-driven β-glucuronidase expression was first observed in meristemoids rather than calli of wheat seeds subjected to anoxia. Taken together, these results indicate that YSK2-type wzy2 can be induced directly by ABA, low temperature, anoxia, and GA treatments and indirectly by drought, implying that different cis-acting elements interact in stress response cross talk.