Determination of L- ascorbic Acid in plasma by voltammetric method

Area of Science:

• Electrochemistry
• Analytical Chemistry
• Biochemistry

Background:

• Voltammetric techniques are crucial for analyzing biological molecules like L-ascorbic acid (AA).
• Direct determination of AA is challenging due to electrode surface issues with conventional materials.
• Electrochemical behavior of AA is influenced by pH and interfering species.

Purpose of the Study:

• To develop and optimize a voltammetric method for L-ascorbic acid (AA) determination.
• To investigate the effects of pH, sweep rate, and interfering substances on AA detection.
• To establish a reliable method for AA quantification in biological samples.

Main Methods:

• Linear-scan voltammetry was employed using a gold electrode.
• Current-voltage (I-E) curves were analyzed at varying pH and sweep rates.
• Copper ions were used as an interference-eliminating agent.

Main Results:

• L-ascorbic acid was accurately quantified in aqueous media within the range of 1-175 μg/mL.
• The optimal conditions were determined as pH 3.2 and a sweep rate of 7500 mV/s.
• A low detection limit of 0.3 μg/mL was achieved, with good linearity (r² = 0.9977) and repeatability.

Conclusions:

• The developed voltammetric method offers a sensitive and selective approach for L-ascorbic acid (AA) analysis.
• The use of copper ions effectively mitigates interference from substances like uric acid and sugars.
• This method provides a robust tool for AA determination in biological contexts.