Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.3K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

16.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
16.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Localizing axial dense emitters based on single-helix point spread function and compressed sensing.

Nanophotonics (Berlin, Germany)·2025
Same author

Polymeric nanoparticles with a thermoresponsive shell loaded with fluorescent molecules allow for thermally enhanced fluorescence imaging and singlet oxygen generation.

Nanoscale advances·2025
Same author

Novel nitrogen-doped carbon dots with triple targetability as a fluorescent probe for bioimaging of living cells.

Analytica chimica acta·2025
Same author

UNET-FLIM: A Deep Learning-Based Lifetime Determination Method Facilitating Real-Time Monitoring of Rapid Lysosomal pH Variations in Living Cells.

Analytical chemistry·2025
Same author

Dynamic Monitoring of Organelle Interactions in Living Cells via Two-Color Digitally Enhanced Stimulated Emission Depletion Super-resolution Microscopy.

The journal of physical chemistry letters·2025
Same author

Magnetic field-induced synergistic therapy of cancer using magnetoplasmonic nanoplatform.

Materials today. Bio·2025
Same journal

<i>In vivo</i> measurement of NADH fluorescence lifetime in skeletal muscle via fiber-coupled time-correlated single photon counting.

Journal of innovative optical health sciences·2025
Same journal

Correlating NAD(P)H lifetime shifts to tamoxifen resistance in breast cancer cells: A metabolic screening study with time-resolved flow cytometry.

Journal of innovative optical health sciences·2025
Same journal

Stick-slip nonuniform rotation distortion correction in distal scanning optical coherence tomography catheters.

Journal of innovative optical health sciences·2024
Same journal

Spatial Sensitivity to Absorption Changes for Various Near-Infrared Spectroscopy Methods: A Compendium Review.

Journal of innovative optical health sciences·2024
Same journal

Optical redox imaging of ANT1-deficient muscles.

Journal of innovative optical health sciences·2024
Same journal

Ring artifacts removal in X-ray-induced acoustic computed tomography.

Journal of innovative optical health sciences·2024
See all related articles

Related Experiment Video

Updated: May 4, 2026

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection
07:42

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection

Published on: February 24, 2026

775

RECENT PROGRESS IN MULTIFOCAL MULTIPHOTON MICROSCOPY.

Junle Qu1, Lixin Liu2, Yonghong Shao1

  • 1College of Optoelectronic Engineering, Shenzhen University, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen 518060, P. R. China.

Journal of Innovative Optical Health Sciences
|December 24, 2013
PubMed
Summary
This summary is machine-generated.

Multifocal multiphoton microscopy (MMM) enhances 3D fluorescence imaging speed and light efficiency. This study explores various MMM setups, including a novel spatial light modulator (SLM) approach.

Keywords:
Multifocal multiphoton microscopy (MMM)beamsplitterdiffractive optical element (DOE)microlens arrayspatial light modulator (SLM)

More Related Videos

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

8.7K
Multiphoton Intravital Imaging for Monitoring Leukocyte Recruitment during Arteriogenesis in a Murine Hindlimb Model
07:50

Multiphoton Intravital Imaging for Monitoring Leukocyte Recruitment during Arteriogenesis in a Murine Hindlimb Model

Published on: September 30, 2021

1.6K

Related Experiment Videos

Last Updated: May 4, 2026

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection
07:42

Super-Resolution Imaging and Shared Management: A Protocol for Confocal Microscopy with Multiplex Detection

Published on: February 24, 2026

775
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

8.7K
Multiphoton Intravital Imaging for Monitoring Leukocyte Recruitment during Arteriogenesis in a Murine Hindlimb Model
07:50

Multiphoton Intravital Imaging for Monitoring Leukocyte Recruitment during Arteriogenesis in a Murine Hindlimb Model

Published on: September 30, 2021

1.6K

Area of Science:

  • Biomedical imaging
  • Optical microscopy
  • Fluorescence imaging

Background:

  • Multifocal multiphoton microscopy (MMM) is crucial for rapid 3D fluorescence imaging in biomedicine.
  • MMM enhances imaging speed and excitation light efficiency by creating multiple focal points for parallel excitation and simultaneous signal detection.

Purpose of the Study:

  • To review and compare different Multifocal Multiphoton Microscopy (MMM) configurations.
  • To present recent advancements in MMM technology, specifically using a spatial light modulator (SLM).

Main Methods:

  • Discussion of various MMM beam-splitting techniques: Nipkow spinning disk, microlens array, beamsplitting mirrors, and diffractive optical elements (DOE).
  • Detailed presentation of a novel MMM system utilizing a spatial light modulator (SLM).

Main Results:

  • Comparison of the performance and features of different MMM setups.
  • Demonstration of the capabilities of the SLM-based MMM system for advanced imaging.

Conclusions:

  • Multifocal multiphoton microscopy (MMM) offers significant advantages for high-speed, efficient 3D fluorescence imaging.
  • Spatial light modulator (SLM) technology represents a promising development for future MMM systems.