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A Magnetic-Bead-Based Mosquito DNA Extraction Protocol for Next-Generation Sequencing
Published on: April 15, 2021
An efficient protocol for total DNA extraction from the members of order Zingiberales- suitable for diverse PCR based
Khumallambam Devala Devi1, Kshetrimayum Punyarani1, Nandeibam Samarjit Singh1
1Medicinal Plants & Horticultural Resources Division, Institute of Bioresources and Sustainable Development, Takyelpat Institutional Area, Imphal, 795001 Manipur India.
Abstract:
Different protocols are usually used for extracting total deoxyribonucleic acid (DNA) from different plant species of same order and DNA of the associated viruses. Here, we describe a rapid, efficient and universal protocol for isolating total DNA from the members of Zingiberales which harbor a high amount of polysaccharides and secondary metabolites. DNA isolated with this protocol was successfully used for PCR based downstream applications viz. random amplified polymorphic DNA (RAPD), Inter-simple sequence repeats (ISSR), DNA barcoding gene (Internal transcribed spacer and trnl-f) amplification and detection of the viruses.
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