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Related Concept Videos

Genome Annotation and Assembly03:36

Genome Annotation and Assembly

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The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
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DNA as a Genetic Template02:05

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Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
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The DNA Helix01:07

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Deoxyribonucleic acid, or DNA, is the genetic material responsible for passing traits from generation to generation in all organisms and most viruses. DNA is composed of two strands of nucleotides that wind around each other to form a spring-like structure called a double helix. However, the double helix is not perfectly symmetrical. Instead, there are regularly occurring grooves in the structure. The major groove occurs where the sugar-phosphate backbones are relatively far apart. This space...
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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Related Experiment Video

Updated: May 4, 2026

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
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Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

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[Perspective on the novel methods for DNA assembly].

Lei Li, Yinhua Lu, Weihong Jiang

    Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
    |December 25, 2013
    PubMed
    Summary

    Synthetic biology has advanced with new DNA assembly methods. This review covers key in vivo and in vitro techniques for building synthetic devices and pathways.

    Area of Science:

    • Synthetic biology
    • Molecular biology
    • Biotechnology

    Context:

    • The advent of synthetic biology, marked by the 2010 artificial synthesis of Mycoplasma mycoides, has been significantly enabled by advancements in DNA assembly.
    • Yeast's DNA recombination capabilities played a crucial role in early synthetic biology breakthroughs.

    Purpose:

    • To review and introduce major in vivo and in vitro DNA assembly protocols.
    • To highlight the evolution of DNA joining and organizational schemes for synthetic biology applications.

    Summary:

    • Discusses in vivo homologous recombination and various in vitro methods like Biobrick/BglBrick, SLIC, and Gibson assembly.
    • Emphasizes that all DNA assembly methods fundamentally rely on DNA joining and organizational strategies.
    • Details how these techniques accelerate the construction of synthetic part libraries, biosynthetic pathways, and microbial chromosomes.

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    Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures
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    Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
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    Impact:

    • Facilitates the construction of diverse synthetic functional devices and complex biosynthetic pathways.
    • Accelerates research and development in synthetic biology, particularly in China.
    • Provides a foundational understanding of DNA assembly for researchers in the field.