[Engineering Saccharomyces cerevisiae for sclareol production].

Area of Science:

• Biotechnology
• Metabolic Engineering
• Natural Product Synthesis

Background:

• Sclareol, a labdane-type diterpene, is primarily utilized as a fragrance ingredient.
• Current production methods for sclareol are limited, necessitating alternative sustainable production strategies.

Purpose of the Study:

• To establish a microbial host for the biosynthesis of sclareol.
• To engineer Saccharomyces cerevisiae for the heterologous production of sclareol.

Main Methods:

• Constructed synthetic pathways by integrating labdenediol diphosphate synthase (LPPS) and terpene synthase (TPS) genes from Salvia sclarea into Saccharomyces cerevisiae.
• Optimized sclareol production through overexpression of precursor biosynthesis enzymes, creation of fusion proteins for substrate channeling, and removal of signal peptides from LPPS and TPS.

Main Results:

• Engineered yeast strain S6 successfully produced sclareol.
• Optimal shake flask conditions yielded 8.96 mg/L of sclareol, demonstrating successful metabolic engineering.

Conclusions:

• The study provides a viable microbial platform for sclareol production.
• These findings offer valuable insights for developing heterologous hosts for terpenoid biosynthesis.
• This approach supports sustainable production of fragrance ingredients.