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Updated: Aug 16, 2026
Microscopy Based Methods for the Assessment of Epithelial Cell Migration During In Vitro Wound Healing
Published on: January 2, 2018
Histological techniques for the study of granular cells during wound healing
Abstract:
We have used GMA-embedded tissues sectioned 2-3 mu for the identification of granulated cells in wounded and normal macaque tissues. Mast cell granules stain brilliantly with Giemsa or with PAS counterstained with alcian blue, neutrophils stand out clearly when treated with the PAS method and macrophage granules stain with PAS as well as with basic dyes. These techniques are helpful for the identification of these cells during wound healing and during inflammatory processes.
Insights
This study details methods for identifying granulated cells in macaque tissues during wound healing. Specific staining techniques differentiate mast cells, neutrophils, and macrophages for better analysis.
Area of Science:
- Histology
- Cell Biology
- Wound Healing Research
Background:
- Granulated cells play crucial roles in tissue repair and inflammation.
- Accurate identification of specific granulated cell types is essential for understanding these processes.
- Existing histological methods may require optimization for clarity in macaque models.
Purpose of the Study:
- To establish reliable histological techniques for identifying granulated cells in macaque tissues.
- To differentiate between mast cells, neutrophils, and macrophages using specific staining protocols.
- To aid in the study of cellular responses during wound healing and inflammation.
Main Methods:
- Utilized GMA (glycol methacrylate) embedding for tissue preparation.
- Sectioned tissues to 2-3 micrometers thickness for detailed analysis.
- Employed Giemsa staining, Periodic Acid-Schiff (PAS) staining, and alcian blue counterstaining.
Main Results:
- Mast cell granules stained effectively with Giemsa or PAS/alcian blue.
- Neutrophils were clearly visualized using the PAS method.
- Macrophage granules demonstrated positive staining with both PAS and basic dyes.
Conclusions:
- The described staining techniques provide effective identification of key granulated cells.
- These methods are valuable tools for investigating cellular dynamics in wound healing.
- The study facilitates research into inflammatory processes in macaque models.
