Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Correlations between cyclic AMP binding and chemoreception in Paramecium.

R Smith, R R Preston, S Schulz

    Biochimica Et Biophysica Acta
    |April 22, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A survey of canine expressed sequence tags and a display of their annotations through a flexible web-based interface.

    The Journal of heredity·2003
    Same author

    K(+)-channel transgenes reduce K(+) currents in Paramecium, probably by a post-translational mechanism.

    Genetics·2001
    Same author

    A two-cell biosensor that couples neuronal cells to optically monitored fish chromatophores.

    Biosensors & bioelectronics·2001
    Same author

    Isolation and characterization of magbane, a magnesium-lethal mutant of paramecium.

    Genetics·2001
    Same author

    A study of the fluorescence of some newly synthesized europium complexes with pyrazolone derivatives.

    Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2001
    Same author

    Genomic organization of human neuropilin-1 and neuropilin-2 genes: identification and distribution of splice variants and soluble isoforms.

    Genomics·2000
    Same journal

    Cumulative Contents.

    Biochimica et biophysica acta·2020
    Same journal

    Molecular Basis of Disease Cumulative Contents.

    Biochimica et biophysica acta·2020
    Same journal

    General Subjects Cumulative Contents.

    Biochimica et biophysica acta·2020
    Same journal

    Erratum to 'on the role of exchangeable hydrogen bonds for the kinetics of P680<sup>+·</sup> Q<sub>A</sub> <sup>-·</sup> formation and P680<sup>+·</sup> Pheo<sup>-·</sup> recombination in photosystem II' [Biochim. Biophys. Acta 1276 (1996) 35-44].

    Biochimica et biophysica acta·2019
    Same journal

    Oligomeric state of the light-harvesting complexes B800-850 and B875 from purple bacterium Rubrivivax gelatinosus in detergent solution.

    Biochimica et biophysica acta·2019
    Same journal

    Regulation of pigment content and enzyme activity in the cyanobacterium Nostoc sp. Mac grown in continuous light, a light-dark photoperiod, or darkness.

    Biochimica et biophysica acta·2019
    See all related articles

    Paramecium tetraurelia are attracted to cyclic adenosine monophosphate (cAMP) because it signals food presence. This attraction is mediated by specific, saturable receptors on the cell surface.

    Area of Science:

    • Cellular Biology
    • Biochemistry
    • Behavioral Ecology

    Background:

    • Paramecium tetraurelia, a single-celled organism, exhibits chemotaxis, a directed movement in response to chemical stimuli.
    • Cyclic adenosine monophosphate (cAMP) is a known signaling molecule in various biological systems.

    Purpose of the Study:

    • To investigate the mechanism underlying Paramecium tetraurelia's attraction to cyclic AMP (cAMP).
    • To determine if cAMP acts as a chemoattractant and to characterize the nature of this interaction.

    Main Methods:

    • Measured the binding of radiolabeled cyclic [3H]AMP to whole Paramecium cells.
    • Employed High-Performance Liquid Chromatography (HPLC) to analyze nucleotide degradation and uptake.
    • Conducted experiments in the presence and absence of IBMX, a phosphodiesterase inhibitor.

    Related Experiment Videos

    Main Results:

    • Cyclic AMP (cAMP) binding to Paramecium cells was found to be specific, saturable, and reversible, indicating receptor-mediated interaction.
    • External cAMP was degraded by Paramecium cells unless a phosphodiesterase inhibitor (IBMX) was present.
    • Small amounts of cAMP were observed to be taken up by the cells.

    Conclusions:

    • The attraction of Paramecium tetraurelia to cyclic AMP (cAMP) is mediated by specific cell surface receptors.
    • Cyclic AMP (cAMP) itself, not its degradation products, serves as the attractant stimulus.
    • The presence of IBMX is crucial for accurate measurement of cAMP binding and attraction studies.