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Related Experiment Video

Updated: May 4, 2026

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

Published on: April 5, 2018

Mining cancer methylomes: prospects and challenges.

Clare Stirzaker¹, Phillippa C Taberlay¹, Aaron L Statham²

¹Epigenetics Program, Garvan Institute of Medical Research, The Kinghorn Cancer Centre, Sydney 2010, NSW, Australia; St Vincent's Clinical School, University of NSW, Sydney 2010, NSW, Australia.

Trends in Genetics : TIG

|December 26, 2013

Summary

Understanding the human methylome requires assessing millions of CpG sites. Genome-wide methods offer insights but often cover less than 5% of all sites, highlighting the need for comprehensive approaches in health and disease research.

Keywords:

DNA methylation cancer methylome epigenetics

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Last Updated: May 4, 2026

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Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer

Published on: September 18, 2020

Area of Science:

Genomics
Epigenetics
Molecular Biology

Background:

The human genome contains over 28 million CpG sites.
DNA methylation plays a crucial role in health and disease.
Understanding the methylome is essential for biological and medical research.

Purpose of the Study:

To discuss various approaches for methylome studies.
To compare the genome coverage of different methylome analysis protocols.
To examine the extent of published cancer methylomes using genome-wide methods.

Main Methods:

Genome-wide analysis using arrays and high-throughput sequencing.
Comparison of promoter, gene, and intergenic region coverage.
Assessment of individual CpG methylation quantitation capacity.

Main Results:

Most commonly used genome-wide methods, excluding whole-genome bisulfite sequencing, detect less than 5% of all CpG sites.
Different protocols offer unique advantages and disadvantages for methylome analysis.
Analysis of published cancer methylomes generated through genome-wide approaches.

Conclusions:

Comprehensive assessment of the methylome is necessary for understanding DNA methylation's role.
Current genome-wide methods have limitations in CpG site coverage.
Further development of methylome analysis techniques is needed for complete understanding.