Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

1.3K
Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exploring gene therapy for developmental and epileptic encephalopathies (DEEs): possibilities or promises?

Regenerative medicine·2026
Same author

Seroprevalence of anti-AAV antibodies in a healthy adult Spanish population: findings from the SAAVIA study.

Gene therapy·2026
Same author

Coronaviruses reprogram the tRNA epitranscriptome to favor viral protein expression.

Nature communications·2026
Same author

Targeting Amyotrophic Lateral Sclerosis with Gene Therapy: From Silencing Genes to Enhancing Neuroprotection.

Human gene therapy·2025
Same author

Long-term effects of s-KL treatment in wild-type mice: Enhancing longevity, physical well-being, and neurological resilience.

Molecular therapy : the journal of the American Society of Gene Therapy·2025
Same author

Gene therapy rescues brain edema and motor function in a mouse model of megalencephalic leukoencephalopathy with subcortical cysts.

Molecular therapy : the journal of the American Society of Gene Therapy·2025

Related Experiment Video

Updated: May 4, 2026

An Efficient Method for Adenovirus Production
10:06

An Efficient Method for Adenovirus Production

Published on: June 10, 2021

16.0K

Helper dependent adenovirus vectors: progress and future prospects.

Dan Cots, Assumpció Bosch, Miguel Chillón1

  • 1Department of Biochemistry and Molecular Biology, 5th floor, Edifici H, Center of Animal Biotechnology and Gene Therapy (CBATEG), Universitat Autònoma de Barcelona, Bellaterra 08193, Spain. miguel.chillon@uab.es.

Current Gene Therapy
|December 27, 2013
PubMed
Summary

Helper-dependent adenovirus (HDAd) vector production has advanced significantly, achieving high titers and low contamination. These improved HDAd vectors offer large capacity and reduced immune response, making them valuable for gene therapy research.

More Related Videos

Cloning and Large-Scale Production of High-Capacity Adenoviral Vectors Based on the Human Adenovirus Type 5
13:17

Cloning and Large-Scale Production of High-Capacity Adenoviral Vectors Based on the Human Adenovirus Type 5

Published on: January 28, 2016

14.8K
Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches
13:36

Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches

Published on: May 6, 2015

10.0K

Related Experiment Videos

Last Updated: May 4, 2026

An Efficient Method for Adenovirus Production
10:06

An Efficient Method for Adenovirus Production

Published on: June 10, 2021

16.0K
Cloning and Large-Scale Production of High-Capacity Adenoviral Vectors Based on the Human Adenovirus Type 5
13:17

Cloning and Large-Scale Production of High-Capacity Adenoviral Vectors Based on the Human Adenovirus Type 5

Published on: January 28, 2016

14.8K
Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches
13:36

Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches

Published on: May 6, 2015

10.0K

Area of Science:

  • Biotechnology
  • Gene Therapy
  • Molecular Virology

Background:

  • Helper-dependent adenovirus (HDAd) vectors are crucial tools in gene therapy and molecular biology research.
  • Previous production systems had limitations in titer and helper contamination.

Purpose of the Study:

  • To review recent advancements in HDAd vector production systems.
  • To highlight current applications of HDAd vectors in research.
  • To discuss the future potential of HDAd vectors in gene therapy.

Main Methods:

  • Review of literature on HDAd vector production and applications.
  • Analysis of recent technological improvements in vector generation.
  • Synthesis of data on vector characteristics and performance.

Main Results:

  • Modern HDAd production systems yield high titers (1 × 10(13) IU/ml) with minimal helper contamination (<0.1%).
  • HDAd vectors possess large insert capacity and elicit lower cellular immune responses compared to first-generation vectors.
  • HDAd vectors are increasingly accessible and utilized in various research fields.

Conclusions:

  • Significant progress in HDAd vector production has enhanced their utility and accessibility.
  • The favorable characteristics of HDAd vectors position them as promising candidates for future gene therapy applications.