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Updated: May 4, 2026

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells
Published on: November 28, 2015
[Transforming growth factor β1 enhances the invasiveness of human glioma cell line via ERK/MAPK pathway]
Lihua Liu1, Qin Dai, Zhigang Min
1Department of Neurology, First Affiliated Hospital of Xi'an Jiaotong University College of Medicine, Xi'an 710061, China.
Objective:
To investigate the effect of transforming growth factor β1 (TGF-β1) on the invasiveness of human glioma SF767 cell line in vitro and explore the possible molecular mechanism.
Methods:
Human glioma SF767 cell line treated with TGF-β1 for 12, 24, or 48 h were examined for the expressions of epithelial-mesenchymal transition- and ERK/MAPK pathway-associated proteins using Western blotting. MTT assay and Transwell assay were employed to assess the changes in the cell proliferation and invasiveness after TGF-β1 treatment, respectively.
Results:
Western blotting showed that TGF-β1 treatment up-regulated the expressions of vimentin, MMP-2, P-ERK, and Zeb-1 and down-regulated E-cadherin expression in SF767 cells. TGF-β1 treatment of the cells resulted in significantly shortened doubling time of cells and obviously increased cell invasiveness.
Conclusion:
TGF-β1 induces epithelial-mesenchymal transition of SF767 cell line to increase its invasiveness possibly via ERK/MAPK pathway.
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