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Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Tandem Mass Spectrometry01:21

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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Mass Spectrometers

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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
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Peptide Identification Using Tandem Mass Spectrometry01:33

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
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Updated: May 4, 2026

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells
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[Latest development in mass spectrometry for clinical application].

Masahiko Takino1

  • 1Agilent Technologies Japan, Ltd. Hachioji 192-8510, Japan. masahiko_takino@agilent.com

Rinsho Byori. the Japanese Journal of Clinical Pathology
|December 28, 2013
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Liquid chromatography-tandem mass spectrometry (LC-MS/MS) enhances clinical chemistry analysis, particularly for low molecular weight biomarkers. This review covers recent LC-MS advancements, ionization techniques, and complementary mass spectrometry approaches.

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Area of Science:

  • Clinical Chemistry
  • Analytical Chemistry
  • Biomarker Analysis

Context:

  • Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is increasingly adopted in specialized clinical chemistry labs.
  • LC-MS/MS offers significant analytical improvements for low molecular weight biomarker detection.

Purpose:

  • To review the current state of mass spectrometry and related techniques for clinical applications.
  • To highlight recent developments and trends in LC-MS technology.

Summary:

  • The review focuses on advancements in LC-MS, including novel ionization techniques and automated online sample preparation.
  • It also discusses ion mobility spectrometry and emerging mass spectrometry methods that complement LC-MS.
  • Key trends in mass spectrometry for clinical chemistry are explored.

Impact:

  • Provides an overview of cutting-edge LC-MS technologies for clinical laboratories.
  • Facilitates the understanding of emerging analytical approaches for biomarker discovery and diagnostics.
  • Supports the advancement of clinical chemistry through improved analytical potential.