[Analysis of the association between cellulose and lignin by carbon 13 tracer method]

In order to further understand the association between cellulose and lignin in gymnosperm plant, carbohydrate part of the lignin-carbohydrate complexes was analyzed. Cellulose precursor, i.e., (6(-13) C) uridine diphoshphate glucose, was synthesized, and injected into a living ginkgo tree with lignin inhibitor AOPP and exogenous lignin precursor. The results from the determination of 13C abundance indicate that the deposition of cellulose in the cell wall is very fast and effective in the primary wall. The analysis of high resolution solid nuclear magnetic 13C NMR spectra confirms that the bonds between C5 position of glucose units in cellulose and alpha-carbons of lignin side chain are benzyl ether linkage.