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Published on: February 16, 2018
Ultrasensitive IL-6 electrochemical immunosensor based on Au nanoparticles-graphene-silica biointerface
Guangfeng Wang1, Xiuping He2, Ling Chen2
1College of Chemistry and Materials Science, Key Laboratory for Functional Molecular Solids of the Education Ministry of China, Anhui Key Laboratory of Chem-Biosensing, Center for Nano Science and Technology, Anhui Normal University, Wuhu 241000, PR China; Anhui Key Laboratory of Controllable Chemistry Reaction & Material Chemical Engineering, HeFei University of Technology, Hefei 230009, PR China.
Abstract:
An Interleukin-6 (IL-6) electrochemical immunosensor was fabricated based on the Au nanoparticles (AuNP)-graphene-silica sol-gel as immobilization biointerface and AuNP-polydopamine (PDA)@carbon nanotubes (CNT) as the label of HRP-bound antibodies. The AuNP-graphene-silica sol-gel film was prepared in situ and modified on the ITO electrode, providing a stable network for the immobilization of antibody and exhibiting a dynamic working range of 1-40 pg/mL with a low detection limit of 0.3 pg/mL IL-6 (at 3s). The results of serum samples with the sensor received an acceptable agreement with the ELISA method. Importantly, this method provided a promising ultrasensitive assay strategy for clinical applications.
