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Thermostable ethanol-insoluble antigens of human heart muscle
Organ-specific reactions were observed between thermostable ethanol-insoluble (BE) fraction of human heart muscle and its corresponding rabbit antiserum by means of tanned cell hemagglutination test, enzyme immunoassay, and gel precipitation test. Two heart-specific components could be identified using gel precipitation and immunoblotting procedures. Antibodies to human heart muscle BE combined also with heart BE preparations from mammals other than man; these interspecies cross-reactions included also rabbit heart BE pointing to the 'auto' nature of some of the antibodies under investigation. The heart antigens under study were distinct from the organ-specific antigen demonstrated by Espinosa and Kaplan in acid extract of human heart muscle.
Organ-specific reactions were observed between thermostable ethanol-insoluble (BE) fraction of human heart muscle and its corresponding rabbit antiserum by means of tanned cell hemagglutination test, enzyme immunoassay, and gel precipitation test. Two heart-specific components could be identified using gel precipitation and immunoblotting procedures. Antibodies to human heart muscle BE combined also with heart BE preparations from mammals other than man; these interspecies cross-reactions included also rabbit heart BE pointing to the 'auto' nature of some of the antibodies under investigation. The heart antigens under study were distinct from the organ-specific antigen demonstrated by Espinosa and Kaplan in acid extract of human heart muscle.