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Related Concept Videos

Patch Clamp01:18

Patch Clamp

6.0K
Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...
6.0K

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Related Experiment Video

Updated: Apr 22, 2026

Single Cell Electroporation in vivo within the Intact Developing Brain
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Single Cell Electroporation in vivo within the Intact Developing Brain

Published on: July 11, 2008

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Two-photon targeted patching and electroporation in vivo.

Michael Häusser, Troy W Margrie

    Cold Spring Harbor Protocols
    |December 28, 2013
    PubMed
    Summary

    Researchers combined patch-clamp electrophysiology with two-photon microscopy to precisely target and record from specific neuron types in vivo. This technique allows for genetic manipulation of identified neuronal populations within the intact nervous system.

    Area of Science:

    • Neuroscience
    • Cell Biology
    • Biophysics

    Background:

    • Targeted electrophysiological recordings in vivo are crucial for understanding neuronal function.
    • Identifying and isolating specific neuron classes within complex neural circuits remains a challenge.

    Purpose of the Study:

    • To describe novel methods for in vivo imaging and electrophysiological recording from genetically identified neurons.
    • To present techniques for targeting rare neuronal subpopulations for both recording and genetic manipulation.

    Main Methods:

    • Combining patch-clamp electrophysiology with two-photon microscopy for targeted in vivo recordings.
    • Utilizing genetically encoded fluorescent proteins (e.g., GFP) or functional indicators for neuron identification.
    • Adapting the technique for wild-type brains using 'shadowpatching' with membrane-impermeable dyes.

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    In vivo Electroporation of Developing Mouse Retina
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    Related Experiment Videos

    Last Updated: Apr 22, 2026

    Single Cell Electroporation in vivo within the Intact Developing Brain
    13:31

    Single Cell Electroporation in vivo within the Intact Developing Brain

    Published on: July 11, 2008

    12.7K
    In vivo Electroporation of Developing Mouse Retina
    05:53

    In vivo Electroporation of Developing Mouse Retina

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    Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices
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    Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices

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  • Integrating single-cell electroporation for genetic manipulation of targeted neurons.

    • Main Results:

    • Successful imaging and recording from the soma and dendrites of specific neuron classes in vivo.
    • Demonstration of 'shadowpatching' for targeting neurons in non-genetically modified brains.
    • Adaptation of methods for genetic manipulation of targeted neuronal subsets.

    Conclusions:

    • These combined techniques enable precise recording and manipulation of genetically, morphologically, and functionally distinct neuronal subsets.
    • The methods facilitate the study of rare neuron populations within the intact nervous system.
    • This approach advances the ability to dissect neural circuit function at the single-cell level.