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Decreased Expression of Type 5 17β-Hydroxysteroid Dehydrogenase (AKR1C3) Protein Identified in Human Diabetic Skin

Moon-Kyun Cho1

  • 1Department of Dermatology, Soonchunhyang University College of Medicine, Seoul, Korea.

Annals of Dermatology
|December 28, 2013
PubMed
Abstract

Insights

Diabetic skin exhibits reduced AKR1C3 protein expression compared to normal skin. This finding suggests a link between AKR1C3, oxidative stress, and altered metabolic states in diabetes.

Area of Science:

  • Biochemistry
  • Dermatology
  • Metabolic Disorders

Background:

  • Diabetes mellitus is marked by chronic hyperglycemia, leading to increased reactive oxygen species (ROS) production.
  • Elevated ROS induces oxidative stress and activates genes linked to oxidative damage.
  • The protein expression of AKR1C3, an oxidative damage-related gene, in human diabetic skin remains unexplored.

Purpose of the Study:

  • To investigate and compare the protein expression levels of AKR1C3 in human diabetic skin versus normal skin tissue.

Main Methods:

  • Western blotting was employed to quantify AKR1C3 protein levels in 6 diabetic and 6 normal skin tissue samples.
  • Immunohistochemical staining was performed to analyze AKR1C3 expression patterns within the diabetic skin samples.

Main Results:

  • Western blotting revealed a significant decrease in AKR1C3 protein expression in diabetic skin compared to normal skin.
  • Immunohistochemical analysis indicated weak AKR1C3 expression across all examined diabetic skin samples.

Conclusions:

  • AKR1C3 protein is significantly downregulated in diabetic skin.
  • The altered metabolic state in diabetes, associated with elevated ROS production, may involve AKR1C3.
Keywords:
AKR1C3Diabetic skinType 5 17 β-hydroxysteroid dehydrogenase

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