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Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
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Stable and sensitive silver surface plasmon resonance imaging sensor using trilayered metallic structures.

Zhiyou Wang1, Zhiqiang Cheng, Vikramjeet Singh

  • 1National Center for NanoScience and Technology, Beijing 100190, People's Republic of China.

Analytical Chemistry
|December 31, 2013
PubMed
Summary

A novel gold-silver-gold trilayered sensor offers enhanced sensitivity and stability for surface plasmon resonance (SPR) applications. This improved SPR sensor chip provides a viable alternative to traditional gold sensors, boosting performance in biomolecular detection.

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Area of Science:

  • Nanotechnology
  • Materials Science
  • Biomedical Engineering

Background:

  • Silver surface plasmon resonance (SPR) sensors offer higher sensitivity than gold sensors but suffer from instability.
  • Conventional gold SPR sensors are stable but less sensitive, limiting practical applications.
  • Existing silver-gold bilayer sensors have not fully addressed stability concerns.

Purpose of the Study:

  • To develop a novel gold-silver-gold trilayered SPR sensor chip with improved stability and sensitivity.
  • To compare the performance of the new trilayered chip against conventional single-layered gold SPR chips.
  • To assess the potential of the new chip for practical biosensing applications.

Main Methods:

  • Fabrication of a gold-silver-gold trilayered SPR sensor chip with an intermediate gold buffer layer.
  • Stability assessment using optical microscopy, atomic force microscopy (AFM), and prolonged agitation in phosphate-buffered saline (PBS).
  • Performance evaluation using large detection area SPR microscopy, including sensitivity, signal-to-noise ratio (S/N) measurements, and protein array fabrication with biomolecular interaction analysis.

Main Results:

  • The gold-silver-gold trilayered chip demonstrated high integrity and stability comparable to single-layered gold SPR chips after prolonged use and chemical exposure.
  • SPR microscopy revealed over 50% increase in sensitivity and S/N ratio compared to single-layered gold SPR chips at 660 nm.
  • Protein array experiments showed consistent kinetic parameters between the trilayered chip and the single gold layered chip when analyzing human IgG interactions.

Conclusions:

  • The proposed gold-silver-gold trilayered SPR sensor chip overcomes the instability issues of silver sensors while retaining high sensitivity.
  • This novel chip design presents a stable and highly sensitive alternative to conventional single gold layered SPR chips.
  • The enhanced sensing performance makes the trilayered chip a promising platform for advanced biosensing and diagnostic applications.