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Updated: May 4, 2026

Author Spotlight: High-Throughput Measurement of Intracellular ROS Levels in Hepatocellular Lines
Published on: January 19, 2024
A small molecule for controlled generation of reactive oxygen species (ROS)
Allimuthu T Dharmaraja1, Harinath Chakrapani
1Indian Institute of Science Education and Research Pune , Dr. Homi Bhabha Road Pashan, Pune 411 008, Maharashtra, India.
Abstract:
Due to the short half-life of reactive oxygen species (ROS) such as a superoxide radical, controlled and localized generation of ROS is challenging. Here, we report a rationally designed small-molecule 1c that generates ROS only when triggered by a bacterial enzyme. We provide evidence for 1c predictably enhancing the intracellular superoxide radical in a model bacterium. Spatiotemporal control over ROS generation offered by 1c should help better understand stress responses in bacteria to increased ROS.
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