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Related Experiment Videos

Functional and antigenic properties of complement receptor type 2, CR2.

K Mitomo, T Fujita, K Iida

    The Journal of Experimental Medicine
    |May 1, 1987
    PubMed
    Summary

    The C3d receptor (CR2) demonstrates functional activity in regulating the complement cascade. This finding supports CR2

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    Area of Science:

    • Immunology
    • Complement System Biology

    Background:

    • The complement system is a crucial part of innate immunity.
    • Regulatory proteins control complement activation.
    • The C3d receptor (CR2) role in complement regulation was previously unclear.

    Purpose of the Study:

    • To investigate the functional activity of purified C3d receptor (CR2).
    • To determine CR2's role in the complement cascade regulation.
    • To compare CR2's antigenic properties with the complement receptor 1 (CR1).

    Main Methods:

    • Examined cofactor activity of CR2 in factor I-mediated cleavage of iC3b.
    • Utilized purified CR2 and C3b inactivator (Factor I).
    • Performed immunoelectroblotting analysis using anti-CR2 and anti-CR1 antibodies.

    Main Results:

    • CR2 exhibited cofactor activity, facilitating the cleavage of membrane-bound iC3b by Factor I.
    • The CR2-mediated release of C3c was inhibited by anti-CR2 monoclonal antibody (OKB7).
    • Immunoelectroblotting revealed antigenic cross-reactivity between CR2 and CR1 (57H antibody).

    Conclusions:

    • CR2 possesses functional activity in regulating the complement cascade.
    • CR2 shares functional and antigenic similarities with CR1.
    • These findings support the classification of CR2 within the gene family of C3- and C4-regulatory proteins.

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