Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fungal Phylum Ascomycota01:28

Fungal Phylum Ascomycota

2.4K
Phylum Ascomycota, a major division within the subkingdom Dikarya, comprises a diverse range of fungal species, including both unicellular yeasts and filamentous molds such as Aspergillus and Penicillium. These fungi thrive in a variety of habitats, from aquatic ecosystems to terrestrial environments, playing crucial ecological and economic roles.Morphology and ReproductionThe defining characteristic of Ascomycetes, commonly referred to as sac fungi, is the ascus—a sac-like structure that...
2.4K
Sputum Studies II: Culture and Sensitivity01:20

Sputum Studies II: Culture and Sensitivity

2.0K
Description
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Non-conventional diagnostic methods for invasive fungal infections.

Expert review of molecular diagnostics·2025
Same author

Interlaboratory reproducibility of a touch-transfer assay for the assessment of antimicrobial surfaces.

The Journal of hospital infection·2023
Same author

Antimicrobial efficacy and inactivation kinetics of a novel LED-based UV-irradiation technology.

The Journal of hospital infection·2023
Same author

Liposomal amphotericin B-the past.

The Journal of antimicrobial chemotherapy·2022
Same author

Etest ECVs/ECOFFs for Detection of Resistance in Prevalent and Three Nonprevalent <i>Candida</i> spp. to Triazoles and Amphotericin B and Aspergillus spp. to Caspofungin: Further Assessment of Modal Variability.

Antimicrobial agents and chemotherapy·2021
Same author

<i>Aspergillus fumigatus</i> and aspergillosis: From basics to clinics.

Studies in mycology·2021
Same journal

Impact of low-dose chest CT and multiplex PCR on antibiotic usage for community-acquired pneumonia: multicentre prospective controlled before-after study with cluster randomisation (CAP-NEXT study).

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2026
Same journal

Clinical Microbiology and Infection: how did we do in 2025?

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2026
Same journal

Automate the work, not the decision: aligning expectations for AI in clinical microbiology.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2026
Same journal

Doxycycline prophylaxis and gonococcal resistance: reassurance, residual signals, and the case for surveillance.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2026
Same journal

Oral metronidazole versus clindamycin to treat bacterial vaginosis in pregnancies at risk for preterm labor.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2026
Same journal

Efficacy and safety of a radiographic severity-stratified all-oral short treatment regimen for multidrug/rifampicin-resistant tuberculosis in China: An open-label, multi-center,randomized controlled, non-inferiority trial.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2026
See all related articles

Related Experiment Video

Updated: May 4, 2026

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure
10:33

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure

Published on: May 27, 2022

6.9K

Susceptibility testing in Aspergillus species complex.

C Lass-Flörl1

  • 1Division of Hygiene and Medical Microbiology, Innsbruck Medical University, Innsbruck, Austria.

Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
|December 31, 2013
PubMed
Summary
This summary is machine-generated.

Standardized methods for Aspergillus susceptibility testing are crucial for guiding antifungal therapy. These breakpoints help clinical laboratories identify and manage emerging resistance in Aspergillus fumigatus infections.

Keywords:
Antifungal susceptibility testingAspergillusECOFFclinical breakpoints

More Related Videos

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis
09:52

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis

Published on: March 9, 2018

12.7K
Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology
08:01

Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology

Published on: March 22, 2012

31.0K

Related Experiment Videos

Last Updated: May 4, 2026

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure
10:33

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure

Published on: May 27, 2022

6.9K
Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis
09:52

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis

Published on: March 9, 2018

12.7K
Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology
08:01

Detection of Invasive Pulmonary Aspergillosis in Haematological Malignancy Patients by using Lateral-flow Technology

Published on: March 22, 2012

31.0K

Area of Science:

  • Mycology
  • Clinical Microbiology
  • Antimicrobial Resistance

Background:

  • Susceptibility testing methods for Aspergillus species are established by expert committees.
  • Antifungal susceptibility testing (AFST) breakpoints are essential for clinical decision-making and drug approval.
  • Emerging resistance in Aspergillus fumigatus necessitates reliable diagnostic tools.

Purpose of the Study:

  • To outline the development and validation of AFST methods for Aspergillus.
  • To explain the process of determining breakpoints for antifungal drugs.
  • To highlight the importance of clinical breakpoints in patient management.

Main Methods:

  • Development and validation of standardized AFST methodologies.
  • Utilizing pharmacokinetic, pharmacodynamic, and MIC distribution data for breakpoint setting.
  • Incorporating epidemiological cut-off values in the breakpoint determination process.

Main Results:

  • Established breakpoints provide a basis for routine clinical laboratory testing.
  • Standardized methods enable consistent interpretation of Aspergillus antifungal susceptibility.
  • Increased reporting of Aspergillus fumigatus resistance observed since standard availability.

Conclusions:

  • Standardized AFST methods and breakpoints are vital for effective antifungal therapy.
  • Clinical breakpoints facilitate appropriate treatment selection for invasive aspergillosis.
  • Ongoing monitoring of Aspergillus resistance patterns is critical.