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Temporal changes in MrgC expression after spinal nerve injury.

S-Q He1, L Han2, Z Li2

  • 1Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University, School of Medicine, Baltimore, MD 21205, USA.

Neuroscience
|December 31, 2013
PubMed
Summary

Mas-related G-protein-coupled receptor subtype C (MrgC) is found in pain-sensing neurons. Nerve injury alters MrgC expression, and Mrg activation may inhibit neuropathic pain maintenance.

Keywords:
MrgCdorsal root ganglionnerve injuryneuropathic pain

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Area of Science:

  • Neuroscience
  • Pain Research
  • Molecular Biology

Background:

  • Mas-related G-protein-coupled receptor subtype C (MrgC) is implicated in pain sensation.
  • The precise distribution of MrgC in dorsal root ganglion (DRG) neuron subpopulations and its response to nerve injury remain unclear due to a lack of selective antibodies.

Purpose of the Study:

  • To investigate the distribution of MrgC in rodent DRG neurons.
  • To examine the time-dependent changes in MrgC expression in injured and uninjured DRG neurons following peripheral nerve injury.
  • To determine the role of Mrgs in neuropathic pain maintenance.

Main Methods:

  • Immunohistochemistry to visualize MrgC distribution in DRG neurons.
  • Quantitative real-time PCR and Western blotting to assess MrgC mRNA and protein levels.
  • Behavioral tests in wild-type and Mrg knockout mice following sciatic nerve injury.

Main Results:

  • MrgC immunoreactivity was observed in both IB4-positive (non-peptidergic) and CGRP-positive (peptidergic) DRG neurons in mice and rats.
  • L5 spinal nerve ligation in rats led to decreased MrgC mRNA and protein levels in the injured L5 DRG but increased levels in the adjacent uninjured L4 DRG at 14 and 30 days post-injury.
  • Mrg knockout mice exhibited a longer duration of mechanical hypersensitivity after chronic constriction injury compared to wild-type mice.

Conclusions:

  • Nerve injury induces differential, time-dependent changes in MrgC expression in injured versus uninjured DRG neurons.
  • Activation of Mas-related G-protein-coupled receptors (Mrgs) appears to play an endogenous inhibitory role in the maintenance of neuropathic pain.