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Updated: Jul 20, 2026

A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening
Published on: October 16, 2015
Study on the interaction between 4-thio-5-methyluridine and human serum albumin by spectroscopy and molecular
Xiaohui Zhang1, Hongxiu Zhai1, Ruiqi Gao1
1College of Environment and Chemical Engineering, Dalian University, Dalian, Liaoning 116622, China.
Abstract:
The interaction between 4-thio-5-methyluridine and human serum albumin (HSA) under simulative physiological conditions has been studied by the methods of fluorescence, UV-VIS absorbance and circular dichroism (CD) spectroscopy. The results show that 4-thio-5-methyluridine has a static fluorescence quenching on human serum albumin (HSA). The main forces of both interactions have typical static interaction from thermodynamic data to the determination. The experimental result was in correspondence with molecular modeling theory.
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