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Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes
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Long noncoding RNAs expression profile of the developing mouse heart.

Jin Gai Zhu1, Ya Hui Shen, Hai Lang Liu

  • 1Department of Pediatrics, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing, 210029, People's Republic of China.

Journal of Cellular Biochemistry
|December 31, 2013
PubMed
Summary

This study profiled long noncoding RNAs (lncRNAs) in fetal mouse hearts across development. Researchers identified 1,237 consistently altered lncRNAs, offering new insights into heart development mechanisms.

Keywords:
BIOINFORMATICSHEART DEVELOPMENTLONG NONCODING RNAMICROARRAY

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Area of Science:

  • RNA Biology
  • Developmental Biology
  • Cardiovascular Research

Background:

  • Long noncoding RNAs (lncRNAs) are increasingly recognized as critical regulators in biological pathways.
  • lncRNAs exhibit specific expression patterns during embryonic and organ development.
  • Understanding lncRNA roles is crucial for advancing RNA biology and developmental studies.

Purpose of the Study:

  • To characterize the lncRNA expression profile in the developing fetal mouse heart.
  • To identify differentially expressed lncRNAs at key developmental stages (E11.5, E14.5, E18.5).
  • To explore potential mechanisms of lncRNA involvement in heart development.

Main Methods:

  • Microarray screening was employed to analyze lncRNA profiles.
  • Gene Ontology and Ingenuity Pathway Analysis were used for functional interpretation.
  • Real-time quantitative reverse transcription PCR (qRT-PCR) validated selected lncRNAs.
  • Bioinformatics analysis was performed to predict target gene interactions.

Main Results:

  • A total of 1,237 lncRNAs showed consistent fold changes across the three developmental time points.
  • Significant alterations in gene functions and pathways related to heart development were identified.
  • One specific lncRNA, AK011347, was predicted to be involved in heart development via the Map3k7 gene.

Conclusions:

  • This study identified novel differentially expressed lncRNAs during fetal mouse heart development.
  • These lncRNAs represent potential key players in the mechanisms of normal heart development.
  • The findings provide a foundation for further research into lncRNA functions in cardiovascular development.