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RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma
Published on: March 11, 2014
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Multicentric human papillomavirus-associated head and neck squamous cell carcinoma
Andrew Caley1, Mererid Evans, Ned Powell
1Department of Clinical Oncology, Velindre Cancer Centre, Cardiff, United Kingdom.
Head & Neck
|December 31, 2013
Summary
Human papillomavirus (HPV)-associated head and neck second primary tumors can appear together or separately, sometimes years apart. HPV-16 is the most common type found in these oropharyngeal and other head and neck cancers.
Area of Science:
- Oncology
- Virology
- Otolaryngology
Background:
- Second primary tumors (SPTs) are a significant concern in head and neck cancer (HNC) management.
- Human papillomavirus (HPV) is an established cause of oropharyngeal cancers.
- The characteristics of HPV-associated HNC SPTs require further elucidation.
Purpose of the Study:
- To describe the clinicopathological features of patients diagnosed with HPV-associated head and neck second primary tumors.
- To analyze the presentation patterns (synchronous vs. metachronous) and HPV genotypes of these SPTs.
Main Methods:
- Retrospective analysis of patients with HPV-associated head and neck SPTs across three institutions.
- HPV detection and characterization using p16 immunohistochemistry (IHC), in situ hybridization (ISH) for high-risk HPV DNA, and polymerase chain reaction (PCR) enzyme immunoassay (EIA) for genotyping.
Main Results:
- Eleven patients with HPV-associated head and neck SPTs were identified.
- Five synchronous and six metachronous SPTs were observed, with metachronous tumors showing a mean interval of 5 years.
- The majority of SPTs (11/13) were oropharyngeal, with HPV-16 being the predominant genotype (9/10).
Conclusions:
- HPV-associated head and neck SPTs can manifest as synchronous or metachronous lesions.
- These tumors can occur outside the oropharynx and may arise after significant time intervals.
- Further research is needed to identify at-risk populations and understand the underlying mechanisms of HPV-driven HNC SPT development.
Keywords:
head and neck squamous cell carcinomahuman papillomavirusmulticentricpharyngealsecond primary tumorMore Related Videos
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