Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

The JAK-STAT Signaling Pathway

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...

T Cell Types and Functions

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...

Inflammatory Response

Inflammatory Response

An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...

Master Transcription Regulators

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

NF-κB-dependent Signaling Pathway

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

GH-resistant (Laron) mice: gene therapy with a liver-specific GH receptor causes unbalanced upregulation of female-biased and growth-related genes.

Frontiers in endocrinology·2026

Same author

Limonene from <i>Citrus unshiu</i> Peel Suppresses Neutrophil Trafficking and Alleviates Acute Lung Injury by Directly Targeting β2 Integrin.

Journal of agricultural and food chemistry·2026

Same author

Tangeretin, a bioactive polymethoxyflavone from immature Citrus aurantium L. fruit, ameliorates experimental colitis by targeting the macrophage β2 integrin Mac-1.

Journal of ethnopharmacology·2026

Same author

A tale of two transferrin receptors.

Blood·2026

Same author

Kaempferol reprograms pro-inflammatory macrophage polarization by targeting the PTGS2-PGE<sub>2</sub> axis: A multi-omics deconvolution of a traditional anti-inflammatory herb pair.

Biochemical pharmacology·2026

Same author

A conserved gut-brain axis underlies the neurobehavioral toxicity of a high-sugar diet: A mechanistic study in Drosophila.

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP·2026

Same journal

Analysis of strength degradation of coal and rock masses and stability of mined areas under long term immersion environment.

PloS one·2026

Same journal

Biogenic Silver-Selenium nanocomposite with anticancer activity and potent efficacy against vancomycin-resistant Staphylococcus aureus.

PloS one·2026

Same journal

Preparation and physicochemical characterization of a biodegradable chitosan/carboxymethyl cellulose hydrogel synthesized in NaOH/urea medium.

PloS one·2026

Same journal

Action-guilt, survivor-guilt, and depression in combat-related PTSD.

PloS one·2026

Same journal

Explainable machine learning for predicting activities of daily living at discharge in stroke patients: A retrospective study using SHAP interpretability.

PloS one·2026

Same journal

Deep learning based two-way feature depiction model for brain tumor detection.

PloS one·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 4, 2026

Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization

Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization

Published on: October 6, 2019

Inflammation regulates TMPRSS6 expression via STAT5.

Delphine Meynard¹, Chia Chi Sun¹, Qifang Wu¹

¹Program in Anemia Signaling Research, Division of Nephrology, Program in Membrane Biology, Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, United States of America.

|December 31, 2013

Summary

This summary is machine-generated.

Inflammation downregulates Transmembrane serine protease 6 (TMPRSS6) expression, impacting iron homeostasis. This occurs via decreased Stat5 phosphorylation, offering a new mechanism for inflammation-induced hepcidin regulation.

More Related Videos

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice

Published on: March 24, 2017

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

Published on: October 9, 2016

Related Experiment Videos

Last Updated: May 4, 2026

Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization

Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization

Published on: October 6, 2019

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice

Published on: March 24, 2017

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

Published on: October 9, 2016

Area of Science:

Molecular biology
Immunology
Iron metabolism

Background:

Transmembrane serine protease 6 (TMPRSS6) regulates iron homeostasis by inhibiting hepcidin.
Hepcidin, a key regulator of iron, is upregulated by inflammation and influences immunity.
The effect of inflammation on TMPRSS6 expression and its regulatory mechanism remained unclear.

Purpose of the Study:

To investigate whether inflammation modulates TMPRSS6 expression.
To elucidate the underlying mechanism of inflammatory regulation of TMPRSS6.
To understand the role of TMPRSS6 in inflammation-induced hepcidin control.

Main Methods:

In vitro study using a human hepatoma cell line treated with interleukin-6.
In vivo study involving lipopolysaccharide injection in mice.
Analysis of TMPRSS6 expression and Stat5 phosphorylation.

Main Results:

Inflammation was found to downregulate TMPRSS6 expression both in vitro and in vivo.
The downregulation of TMPRSS6 by inflammation in mice was independent of the Bmp-Smad pathway.
Inflammation decreased Stat5 phosphorylation, and Stat5 was identified as a direct positive regulator of TMPRSS6 expression via promoter binding.

Conclusions:

Inflammation downregulates TMPRSS6 expression through decreased Stat5 phosphorylation.
Stat5 directly binds to the TMPRSS6 promoter, positively regulating its expression.
This inflammatory modulation of TMPRSS6, via Stat5, provides an additional mechanism for inflammation-driven hepcidin upregulation, affecting iron homeostasis and immunity.