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Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy
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Detecting plasmon resonance energy transfer with differential interference contrast microscopy.

Ashley E Augspurger1, Anthony S Stender, Rui Han

  • 1Department of Chemistry, Iowa State University & The Ames Laboratory , U.S. Department of Energy, Ames, Iowa 50011, United States.

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Summary

Gold nanoparticles enable real-time monitoring of Plasmon Resonance Energy Transfer (PRET) at the single-particle level. This study demonstrates PRET

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Area of Science:

  • Nanotechnology
  • Biophysics
  • Cell Biology

Background:

  • Gold nanoparticles possess unique plasmonic properties, making them suitable for probing intracellular environments and energy transfer.
  • Plasmon Resonance Energy Transfer (PRET) involves energy transfer from a plasmonic nanoparticle donor to an acceptor molecule, detectable via nanoparticle quenching.

Purpose of the Study:

  • To investigate Plasmon Resonance Energy Transfer (PRET) using gold nanospheres as donors and cytochrome c as acceptors.
  • To monitor PRET dynamics in real-time at the single-particle level.
  • To assess the reversibility of PRET and its occurrence within living cells.

Main Methods:

  • Utilized gold nanospheres as plasmonic donors and cytochrome c as acceptor molecules.
  • Employed Differential Interference Contrast (DIC) microscopy for simultaneous observation of nanoparticles and cellular environments.
  • Integrated specially designed microfluidic channels for controlled experiments.

Main Results:

  • Successfully monitored single gold nanoparticle PRET in real-time using DIC microscopy.
  • Demonstrated the reversibility of PRET by introducing phosphate-buffered saline.
  • Observed PRET in gold nanoparticles internalized by HeLa cells during ethanol-induced apoptosis.

Conclusions:

  • Single-particle PRET monitoring is feasible using gold nanoparticles and DIC microscopy.
  • PRET dynamics are observable and reversible in controlled microfluidic environments.
  • Gold nanoparticle-mediated PRET can be detected in living cells undergoing apoptosis, offering insights into cellular processes.