Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Regulatory volume decrease in perfused proximal nephron: evidence for a dumping of cell K+.

K L Kirk, D R DiBona, J A Schafer

    The American Journal of Physiology
    |May 1, 1987
    PubMed
    Summary

    Regulatory volume decrease in kidney proximal nephrons involves a swelling-activated loss of potassium salt and water. Inhibiting the Na+-K+ pump or blocking K+ channels reduces this volume regulation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The American Orthopaedic Association's Own the Bone® database: a national quality improvement project for the treatment of bone health in fragility fracture patients.

    Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2018
    Same author

    A PROSTHETIC GROUP FOR THE RAPID INTRODUCTION OF FLUORINE INTO PEPTIDES AND FUNCTIONALIZED DRUGS.

    Journal of fluorine chemistry·2015
    Same author

    Clarification of the intercellular space phenomenon in toad urinary bladder.

    The Journal of membrane biology·2013
    Same author

    Measurement of the composition of epithelial cells from the toad urinary bladder.

    The Journal of membrane biology·2013
    Same author

    The cellular specificity of the effect of vasopressin on toad urinary bladder.

    The Journal of membrane biology·2013
    Same author

    Invited article: improving safety for the neurologic patient: evaluating medications, literacy, and abuse.

    Neurology·2010

    Area of Science:

    • Nephrology
    • Cell Physiology
    • Renal Tubule Function

    Background:

    • Hyposmotic cell swelling is a critical challenge for kidney proximal nephrons.
    • Understanding the mechanisms of regulatory volume decrease (RVD) is vital for renal health.

    Purpose of the Study:

    • To investigate the role of potassium salt (K-salt) efflux in reversing hyposmotic cell swelling in the perfused proximal nephron.
    • To identify the specific ion transport mechanisms involved in RVD.

    Main Methods:

    • Microscopic and morphometric analyses of perfused proximal nephrons.
    • Experimental manipulation of basolateral membrane K+ chemical potential difference.
    • Inhibition of the Na+-K+ pump (ouabain) and K+ channel blockade (quinine).
    • Assessment of cell volume changes in response to osmotic and ionic perturbations.

    Related Experiment Videos

    Main Results:

    • Inhibiting the Na+-K+ pump or increasing peritubular K+ concentration attenuated the rate of RVD.
    • Peritubular quinine significantly inhibited RVD, indicating the involvement of K+ channels.
    • Exposure to hyposmotic solutions increased basolateral membrane K+ permeability in a quinine-sensitive manner.

    Conclusions:

    • Swelling-activated K-salt loss contributes significantly to the reversal of hyposmotic cell swelling in proximal nephrons.
    • K+ channels and the electrochemical gradient for K+ across the basolateral membrane are crucial for RVD.