Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The lipedema common case report form as a research tool: standardizing lipedema data collection.

Frontiers in global women's health·2026
Same author

Leveraging factors that control alveolar epithelial cell fate enables large-scale expansion for lung tissue engineering.

The Journal of clinical investigation·2026
Same author

Evolving Landscape of Glioblastoma Research: Integrating Therapeutic Advances and Diagnostic Frontiers.

Brain sciences·2026
Same author

Editorial: Role of induced pluripotent stem cells (iPSCs) in regenerative medicine, disease modeling and drug discovery.

Frontiers in bioengineering and biotechnology·2025
Same author

Oncogenic effects of ECM remodeling in obesity and breast cancer.

Oncogene·2025
Same author

Adipose Stromal Cell Heterogeneity and Trafficking: Implications for Breast Cancer Metastasis and Obesity.

Frontiers in bioscience (Landmark edition)·2025

Related Experiment Video

Updated: May 4, 2026

Nonhuman Primate Lung Decellularization and Recellularization Using a Specialized Large-organ Bioreactor
16:45

Nonhuman Primate Lung Decellularization and Recellularization Using a Specialized Large-organ Bioreactor

Published on: December 15, 2013

9.2K

Nonhuman primate lung decellularization and recellularization using a specialized large-organ bioreactor.

Ryan W Bonvillain1, Michelle E Scarritt, Nicholas C Pashos

  • 1Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine.

Journal of Visualized Experiments : Jove
|January 1, 2014
PubMed
Summary

Researchers developed a method to decellularize nonhuman primate lungs, creating scaffolds for bioartificial lung regeneration. This approach, using rhesus macaque lungs, advances regenerative medicine for organ transplantation needs.

More Related Videos

Procedure for Lung Engineering
12:50

Procedure for Lung Engineering

Published on: March 8, 2011

46.5K
Transplantation of Bioengineered Lung Using Decellularized Mouse Lungs and Primary Human Endothelial Cells
10:13

Transplantation of Bioengineered Lung Using Decellularized Mouse Lungs and Primary Human Endothelial Cells

Published on: March 28, 2025

1.0K

Related Experiment Videos

Last Updated: May 4, 2026

Nonhuman Primate Lung Decellularization and Recellularization Using a Specialized Large-organ Bioreactor
16:45

Nonhuman Primate Lung Decellularization and Recellularization Using a Specialized Large-organ Bioreactor

Published on: December 15, 2013

9.2K
Procedure for Lung Engineering
12:50

Procedure for Lung Engineering

Published on: March 8, 2011

46.5K
Transplantation of Bioengineered Lung Using Decellularized Mouse Lungs and Primary Human Endothelial Cells
10:13

Transplantation of Bioengineered Lung Using Decellularized Mouse Lungs and Primary Human Endothelial Cells

Published on: March 28, 2025

1.0K

Area of Science:

  • Regenerative Medicine
  • Tissue Engineering
  • Organ Transplantation

Background:

  • Insufficient donor lungs for transplantation create a critical need for alternative solutions.
  • Bioartificial tissue regeneration using extracellular matrix (ECM) scaffolds offers a promising alternative.
  • Decellularization removes cellular components, preserving the ECM for recellularization and tissue regrowth.

Purpose of the Study:

  • To present protocols for decellularizing nonhuman primate (NHP) lungs.
  • To investigate the potential of NHP lungs as scaffolds for bioartificial lung development.
  • To establish methods for recellularizing and culturing these scaffolds for future clinical applications.

Main Methods:

  • Developed protocols for complete decellularization of rhesus macaque lungs.
  • Isolated the natural lung extracellular matrix (ECM) using detergents, salts, and enzymes.
  • Seeded acellular lungs with mesenchymal stem cells and endothelial cells.
  • Utilized a bioreactor system with mechanical ventilation and pulsatile perfusion for cell culture.

Main Results:

  • Successfully achieved complete decellularization of NHP lungs, preserving ECM structure.
  • Demonstrated the feasibility of seeding acellular lungs with various cell types.
  • Established a bioreactor system to culture cell-seeded lungs under physiological conditions.

Conclusions:

  • Nonhuman primate lungs provide a suitable model for developing bioartificial lungs.
  • Decellularization and recellularization protocols are crucial steps towards regenerative lung therapies.
  • This research advances the field of bioartificial organ development for transplantation.