Related Experiment Video
Updated: May 4, 2026

An Integrated Approach for Microprotein Identification and Sequence Analysis
Published on: July 12, 2022
Gene structure, immune response and evolution: comparative analysis of three 2-Cys peroxiredoxin members of miiuy
Liping Ren1, Yuena Sun1, Rixin Wang1
1Laboratory of Fish Biogenetics and Immune Evolution, College of Marine Science, Zhejiang Ocean University, Zhoushan 316000, China.
Abstract:
Peroxiredoxin family was a superfamily of selenium independent peroxidases. It was divided into six subtypes: Prx1-4 (typical 2-Cys), Prx5 (atypical 2-Cys) and Prx6 (1-Cys). This study reports the isolation and characterization three 2-Cys peroxiredoxin members of full cDNA and genomic clones from miiuy croaker (Miichthys miiuy). The genetic structure analysis showed that the C-terminal catalytic Cys positioned within GEVCPAXW. This sequence was different between Prx3 and Prx4, but was conservative in different species of the same gene, the X base was S in Prx3 but G in Prx4. Tissues expression analysis showed that the expressions of Prx3 in liver and brain were much higher than other tissues; the values of Prx4 in spleen, intestine and kidney were significantly higher than others; and the expression of Prx5 in muscle was higher than that of other tissues. Real-time PCR results showed that there were highest values of these three Prxs emerging with the time post challenge of Vibrio anguillarum in liver, spleen and kidney although the highest value time differed from each other and the expression of these three genes also changed with the change of infection time. These results indicated that expression analysis of these three genes play some positive function against pathogenic bacteria infection in miiuy croaker.
Related Concept Videos
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon...
Gene Families
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
Cis-regulatory Sequences
Gene Duplication and Divergence
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are...
Evolutionary Relationships through Genome Comparisons
Convergent Evolution

