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Mycobacterium tuberculosis DNA detection using surface plasmon resonance modulated by telecommunication wavelength
Shih-Hsiang Hsu1, Yan-Yu Lin2, Shao-Hsi Lu3
1Department of Electronic Engineering, National Taiwan University of Science and Technology, No. 43, Sec. 4, Keelung Rd., Taipei 10607, Taiwan. shsu@mail.ntust.edu.tw.
Sensors (Basel, Switzerland)
|January 1, 2014
Summary
A new surface plasmon resonance sensor detects Mycobacterium tuberculosis (MTB) DNA using stable optical fiber lasers. This biosensor achieves high sensitivity, outperforming commercial equipment for rapid TB diagnosis.
Area of Science:
- Biomedical Engineering
- Optoelectronics
- Molecular Diagnostics
Background:
- Mycobacterium tuberculosis (MTB) infection remains a significant global health challenge.
- Accurate and rapid DNA detection is crucial for timely diagnosis and treatment of TB.
- Existing biosensor technologies face limitations in sensitivity and cost-effectiveness.
Purpose of the Study:
- To develop a novel surface plasmon resonance (SPR) sensor for detecting Mycobacterium tuberculosis (MTB) DNA.
- To utilize repeatable telecommunication wavelength modulation for enhanced sensor performance.
- To achieve high sensitivity and specificity for MTB DNA detection.
Main Methods:
- Fabrication of an SPR sensor utilizing optical fiber communication laser wavelength and stability.
- Employing telecommunication wavelength modulation for signal detection.
- Quantifying MTB DNA concentrations and assessing sensor response characteristics.
Main Results:
- Demonstrated successful detection of MTB DNA at concentrations of 1 μg/mL and 10 μg/mL.
- Achieved consistent spectral half-width in the dip for optimum coupling across telecommunication wavelengths.
- Reported a sensitivity of -0.087 dB/(μg/mL) and a highest sensitivity of 115 ng/mL without DNA immobilization, surpassing the 400 ng/mL limit of commercial equipment.
Conclusions:
- The developed SPR sensor offers a sensitive and efficient method for MTB DNA detection.
- The use of telecommunication wavelength modulation enhances sensor performance and repeatability.
- This technology presents a promising alternative to current commercial biosensors for TB diagnostics.
