Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Urlárins, chlorinated bis-indoles from a benthic mat of cyanobacteria in the West of Ireland.

Phytochemistry·2026
Same author

Neuroprotective Effects of Furanoditerpenes from <i>Spongia (Spongia) Tubulifera</i> through Cyclophilin D Modulation against Ischemia/Reperfusion Injury in BV2 Microglial Cells.

ACS chemical neuroscience·2026
Same author

Okadaic acid triggers NFκB and STAT3 phosphorylation followed by a release of inflammatory markers in human and mouse endothelial cells.

Archives of toxicology·2026
Same author

Macrophages signal cross-talks under hyperglycemic conditions and their link to cyclophilins.

Molecular medicine (Cambridge, Mass.)·2026
Same author

Human TRPV1 Channels are Functional Allosteric Receptors for Ciguatoxins and Brevetoxins.

ACS chemical neuroscience·2025
Same author

Acute toxicity assessment of diarrhetic shellfish toxins by voluntary feeding in mice.

Ecotoxicology and environmental safety·2025

Related Experiment Video

Updated: May 4, 2026

Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance
10:41

Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance

Published on: January 3, 2012

12.8K

Surface plasmon resonance biosensor method for palytoxin detection based on Na+,K+-ATPase affinity.

Amparo Alfonso1, María-José Pazos2, Andrea Fernández-Araujo1

  • 1Department of Pharmacology, Veterinary School, University of Santiago de Compostela, 27002 Lugo, Spain. andrea.fernandez.araujo@rai.usc.es.

Toxins
|January 1, 2014
PubMed
Summary

A new method detects palytoxin (PLTX) by monitoring its binding to the Na+,K+-ATPase using surface plasmon resonance (SPR). This approach offers a sensitive and label-free way to identify PLTX in marine products, crucial for public health.

More Related Videos

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
09:09

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

8.3K
Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR
09:35

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR

Published on: November 29, 2014

22.6K

Related Experiment Videos

Last Updated: May 4, 2026

Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance
10:41

Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance

Published on: January 3, 2012

12.8K
A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
09:09

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

8.3K
Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR
09:35

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance SPR

Published on: November 29, 2014

22.6K

Area of Science:

  • Marine Biology
  • Biochemistry
  • Analytical Chemistry

Background:

  • Palytoxin (PLTX) is a potent marine toxin produced by dinoflagellates, posing a risk through contaminated seafood.
  • The mammalian Na+,K+-ATPase is identified as a high-affinity cellular receptor for PLTX.
  • PLTX disrupts ion transport by converting the Na+,K+-ATPase pump into an open channel.

Purpose of the Study:

  • To develop a novel, label-free detection method for palytoxin (PLTX).
  • To utilize the specific interaction between PLTX and Na+,K+-ATPase for toxin quantification.
  • To provide a sensitive and efficient tool for monitoring PLTX in marine products.

Main Methods:

  • Development of a detection assay based on the binding interaction between PLTX and immobilized Na+,K+-ATPase.
  • Application of Surface Plasmon Resonance (SPR) to monitor the real-time biomolecular reaction without component labeling.
  • Quantification of PLTX-Na+,K+-ATPase binding kinetics by measuring binding rate constants (Kobs) at varying toxin concentrations.

Main Results:

  • The kinetic equilibrium dissociation constant (K(D)) for the PLTX-Na+,K+-ATPase association was determined to be 6.38 × 10-7 ± 6.67 × 10-8 M.
  • The SPR-based method demonstrated effective quantification of PLTX concentration through its binding affinity to Na+,K+-ATPase.
  • The study successfully established a new approach for detecting PLTX-like compounds.

Conclusions:

  • The developed SPR method provides a sensitive and efficient means for detecting palytoxin (PLTX) in marine samples.
  • This label-free technique leverages the toxin's mechanism of action, offering an alternative to expensive and animal-based assays.
  • The findings contribute to enhanced food safety by enabling easier detection of harmful marine toxins.