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Updated: May 4, 2026

Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases
Published on: January 1, 2016
Disruption of biofilm formation by the human pathogen Acinetobacter baumannii using engineered quorum-quenching
Jeng Yeong Chow1, Yuanyong Yang, Song Buck Tay
1Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
Abstract:
Acinetobacter baumannii is a major human pathogen associated with multidrug-resistant nosocomial infections; its virulence is attributed to quorum-sensing-mediated biofilm formation, and disruption of biofilm formation is an attractive antivirulence strategy. Here, we report the first successful demonstration of biofilm disruption in a clinical isolate of A. baumannii S1, using a quorum-quenching lactonase obtained by directed evolution; this engineered lactonase significantly reduced the biomass of A. baumannii-associated biofilms, demonstrating the utility of this antivirulence strategy.
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