Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Association between red blood cell distribution width-to-albumin ratio with erectile dysfunction in US men: Insights from national cross-sectional study in NHANES 2001 to 2004.

Medicine·2026

Same author

A Multinational, Retrospective, Real-World Study: Treatment Patterns in Patients With HER2-Positive Locally Advanced or Metastatic Gastric or Gastroesophageal Junction Adenocarcinoma Beyond First-line in East Asia (HER2+ GASTA Study).

Cancer research and treatment·2026

Same author

Molecular characterisation and phylogenetic placement of a new cestode, <i>Oochoristica turpanoeremiadis</i> sp. nov. (Cestoda: Cyclophyllidea: Linstowiidae), parasitising <i>Eremias roborowskii</i> from Turpan Basin in China.

Parasitology·2026

Same author

Inhibiting METTL3 synergizes with Notch blockade to treat ESCC by targeting cancer stemness via m6A-JAG2.

Journal of gastroenterology·2026

Same author

Association between visceral adiposity index and arteriogenic erectile dysfunction: a multicenter cross-sectional study.

Sexual medicine·2026

Same author

Perioperative serplulimab with neoadjuvant chemotherapy versus perioperative chemotherapy in PD-L1-positive gastric cancer (ASTRUM-006): a randomised, double-blind, multicentre, phase 3 study.

Lancet (London, England)·2026

Search research articles

Related Experiment Video

Updated: May 4, 2026

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

Published on: November 11, 2014

RNA-Seq technology and its application in fish transcriptomics.

Xi Qian¹, Yi Ba, Qianfeng Zhuang

¹1 Department of Animal Science, University of Vermont , Burlington, Vermont.

Omics : a Journal of Integrative Biology

|January 2, 2014

Summary

RNA sequencing (RNA-seq) offers a powerful approach to study fish transcriptomes, overcoming limitations of older methods. This technology advances our understanding of fish biology, from development to stress responses.

More Related Videos

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Published on: October 27, 2017

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets

Published on: February 23, 2024

Related Experiment Videos

Last Updated: May 4, 2026

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

Published on: November 11, 2014

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Published on: October 27, 2017

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets

Author Spotlight: Cost-Effective Transcriptomic Drug Screening - Unlocking New Targets

Published on: February 23, 2024

Area of Science:

Genomics
Transcriptomics
Molecular Biology

Background:

High-throughput sequencing, or next-generation sequencing (NGS), has transformed genomic research.
RNA sequencing (RNA-seq) extends NGS to analyze the dynamic transcriptome, surpassing limitations of microarrays and tag-based methods.
RNA-seq provides unprecedented insights into eukaryotic transcriptomes.

Purpose of the Study:

To review the RNA sequencing (RNA-seq) methodology for fish transcriptomics.
To summarize recent biological discoveries in fish using RNA-seq.
To highlight the impact of RNA-seq on understanding fish biology.

Main Methods:

Overview of RNA-seq workflow, including library construction.
Detailed explanation of bioinformatic analysis pipelines for RNA-seq data.
Synthesis of findings from diverse fish RNA-seq studies.

Main Results:

RNA-seq has significantly advanced the mapping and annotation of fish transcriptomes.
Studies reveal RNA-seq's utility in understanding fish development, adaptive evolution, immunity, and stress responses.
A broad range of fish species, both model and non-model, have benefited from RNA-seq.

Conclusions:

RNA sequencing is a transformative technology for fish transcriptomics.
RNA-seq facilitates comprehensive analysis of biological processes in various fish species.
This review consolidates current knowledge and future directions in fish RNA-seq research.