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Updated: May 4, 2026

Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis
Published on: February 5, 2014
Rapid identification of linezolid resistance in Enterococcus spp. based on high-resolution melting analysis
María Alonso1, Mercedes Marín1, Cristina Iglesias1
1Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain; CIBER Enfermedades Respiratorias-CIBERES (CD06/06/0058), Spain.
Abstract:
The polymorphism G2576T in the 23S rRNA gene is the mutation most frequently associated with linezolid resistance in Enterococcus spp. isolates. We designed a high-resolution melting procedure to detect G2576T which succeeded in identifying linezolid resistant isolates and classified them according to the number of 23S rRNA copies mutated.

