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Detection of retinoid receptors in non-neoplastic canine lymph nodes and in lymphoma
Carlos H de Mello Souza1, Victor E O Valli1, Barbara E Kitchell1
1Department of Small Animal Clinical Sciences, University of Florida, College of Veterinary Medicine, Gainesville, Florida 32610-0126, USA (de Mello Souza); VDx Veterinary Diagnostics. 2019 Anderson Road, Suite C, Davis, California 95616, USA (Valli); Center for Comparative Oncology. D208 Veterinary Medicine Center, Michigan State University, College of Veterinary Medicine, East Lansing, Michigan 48824, USA (Kitchell).
Abstract:
This study evaluated the difference in retinoid receptor expression between non-neoplastic lymph nodes and nodal lymphoma in dogs. Retinoid receptor expression was evaluated by immunohistochemistry in 32 canine lymph nodes. The lymph nodes had been previously diagnosed as non-neoplastic (6 normal and 7 hyperplastic lymph nodes) and B- and T-cell lymphoma (19 cases). Immunohistochemistry for retinoic acid receptors and retinoid-X receptors (and their subtypes α, β, and γ) was performed in all cases. In addition, immunohistochemistry for CD3 and CD79a was performed in all lymphoma cases. Non-neoplastic lymphocytes were negative for all retinoid receptors. Retinoic acid receptor-γ was detected in 100% of B-cell lymphoma and 78% of T-cell lymphoma, while retinoid X receptor-γ was positive in 78% of T-cell lymphoma cases. When normal lymph node architecture was still present, a contrast between retinoid-negative benign cells and retinoid-positive malignant cells was clear. Retinoid receptors were expressed in neoplastic, but not in benign lymphocytes, suggesting their value for both diagnosis and treatment of canine lymphoma.
