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Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity
Published on: March 31, 2021
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Comprehensive proteomic profiling of outer membrane vesicles from Campylobacter jejuni
Kyoung-Soon Jang1, Michael J Sweredoski2, Robert L J Graham2
1Division of Chemistry and Chemical Engineering, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA.
Journal of Proteomics
|January 3, 2014
Summary
Outer membrane vesicles (OMVs) from Campylobacter jejuni contain virulence factors and essential proteins. This study reveals their proteomic profile and potential role in bacterial communication and pathogenesis.
Area of Science:
- Microbiology
- Bacteriology
- Proteomics
Background:
- Gram-negative bacteria release outer membrane vesicles (OMVs) crucial for survival and pathogenesis.
- Campylobacter jejuni is a significant human gastrointestinal pathogen.
Purpose of the Study:
- To perform a comprehensive proteomic analysis of OMVs from Campylobacter jejuni NCTC11168.
- To elucidate the role of OMVs in bacterial infections and communication.
Main Methods:
- Isolation and characterization of OMVs using electron microscopy and dynamic light scattering.
- High-resolution mass spectrometry (LTQ-Orbitrap) for proteomic analysis.
- Bioinformatics analysis for functional enrichment and pathway prediction.
Main Results:
- Identified 134 unique proteins in C. jejuni OMVs.
- Discovered virulence factors and essential proteins for bacterial survival.
- Found enrichment of redox enzymes and implicated the twin-arginine translocation (Tat) pathway in OMV biogenesis.
- Identified N-glycoproteins, suggesting OMVs deliver biologically relevant molecules.
Conclusions:
- Understanding the OMV proteome is vital for studying Campylobacter jejuni.
- OMVs carry virulence factors and proteins essential for survival.
- OMVs may play a role in bacterial communication and pathogenesis, potentially delivering N-linked glycoproteins.
Keywords:
C. jejuni NCTC11168Functional annotationOuter membrane vesicleSignal peptideVirulence factorMore Related Videos
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