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Updated: May 4, 2026

14:43
Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
Published on: August 27, 2014
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Probing physical properties of a DNA-protein complex using nanofluidic channels
Karolin Frykholm1, Mohammadreza Alizadehheidari, Joachim Fritzsche
1Department of Chemical and Biological Engineering, Chalmers University of Technology, SE-412 96, Gothenburg, Sweden.
Small (Weinheim an Der Bergstrasse, Germany)
|January 3, 2014
Abstract:
A method to investigate physical properties of a DNA-protein complex in solution is demonstrated. By using tapered nanochannels and lipid passivation the persistence length of a RecA filament formed on double-stranded DNA is determined to 1.15 μm, in agreement with the literature, without attaching protein or DNA to any handles or surfaces.

