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miR-145 functions as tumor suppressor and targets two oncogenes, ANGPT2 and NEDD9, in renal cell carcinoma

Ruijing Lu1, Ziliang Ji, Xiaoqing Li

  • 1Department of Clinical Laboratory, Peking University Shenzhen Hospital, Shenzhen, 518036, China.

Journal of Cancer Research and Clinical Oncology
|January 4, 2014
PubMed
Abstract

Insights

MicroRNA-145 (miR-145) acts as a tumor suppressor in renal cell carcinoma (RCC). Restoring miR-145 inhibits RCC cell growth, migration, and invasion, offering a potential therapeutic target for this cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Aberrant microRNA (miRNA) expression is implicated in human cancer development.
  • Renal cell carcinoma (RCC) is a significant malignancy with complex molecular underpinnings.
  • Identifying novel tumor suppressors is crucial for understanding RCC pathogenesis and developing targeted therapies.

Purpose of the Study:

  • To identify a novel tumor suppressor miRNA in renal cell carcinoma (RCC).
  • To elucidate the physiological function and molecular mechanisms of miR-145 in RCC.
  • To evaluate miR-145 as a potential therapeutic target for RCC.

Main Methods:

  • Quantitative RT-PCR was used to assess miR-145 expression in RCC and adjacent normal tissues.
  • In vitro assays (proliferation, migration, invasion, apoptosis, cell cycle) were performed to determine miR-145 function.
  • Luciferase reporter assays identified direct targets of miR-145, including oncogenes ANGPT2 and NEDD9.
  • RT(2) Profiler EMT PCR array analyzed the impact of miR-145 on epithelial-to-mesenchymal transition (EMT)-related genes.

Main Results:

  • miR-145 expression was significantly downregulated in RCC tissues compared to normal adjacent tissues.
  • Restoration of miR-145 suppressed RCC cell proliferation, migration, and invasion.
  • miR-145 induced apoptosis and G2-phase cell cycle arrest in RCC cell lines.
  • miR-145 targets oncogenes ANGPT2 and NEDD9 and regulates multiple EMT-associated genes.

Conclusions:

  • miR-145 functions as a tumor suppressor in renal cell carcinoma.
  • The findings suggest that miR-145 holds potential as a therapeutic target for RCC treatment.

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