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Related Concept Videos

Patch Clamp01:18

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

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Related Experiment Video

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Whole-Cell Recording of Calcium Release-Activated Calcium (CRAC) Currents in Human T Lymphocytes
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A computer method for the acquisition and analysis of patch-clamp single-channel currents.

F V McCann, G R Stibitz, T M Keller

    Journal of Neuroscience Methods
    |May 1, 1987
    PubMed
    Summary

    This study introduces a computer-driven method for analyzing channel current data, enabling rapid, interactive analysis of digitized membrane voltage data. The efficient data acquisition and analysis streamline electrophysiological research.

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    Whole-Cell Recording of Calcium Release-Activated Calcium (CRAC) Currents in Human T Lymphocytes
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    Published on: December 21, 2010

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    One-channel Cell-attached Patch-clamp Recording

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    Area of Science:

    • Biophysics
    • Computational Biology
    • Electrophysiology

    Background:

    • Analysis of channel current data is crucial in biophysical research.
    • Traditional methods can be time-consuming and less interactive.
    • Efficient data acquisition and storage are essential for large datasets.

    Purpose of the Study:

    • To present a novel computer-instructed method for analyzing channel current data.
    • To enable rapid, interactive analysis of digitized membrane voltage and channel current data.
    • To facilitate compact storage and identification of electrophysiological records.

    Main Methods:

    • Programming computer systems to deliver sets of programmed membrane voltages.
    • Acquisition of large amounts of digitized channel current data.
    • Utilizing standard computer storage devices for data management.

    Main Results:

    • The method allows for rapid, interactive analysis of channel current data.
    • A typical analysis cycle (10s data at 5 kHz) takes 5-20 minutes.
    • The procedure supports compact data storage and easy record identification.

    Conclusions:

    • The presented method significantly enhances the efficiency of channel current data analysis.
    • This approach is compatible with existing storage protocols and offers benefits over them.
    • The developed programs are available to researchers, promoting wider adoption.