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Updated: May 4, 2026
Intestinal Epithelial Regeneration in Response to Ionizing Irradiation
Published on: July 27, 2022
Gene expression changes after ionizing radiation in endothelial cells derived from human endometrial
Ting Liu1, Xuelian Du, Xiugui Sheng
1Department of Gynecologic Oncology, Shandong Cancer Hospital, Jinan, 250117, Shandong, People's Republic of China.
Background:
Accumulating evidence has demonstrated that death of microvascular endothelial cells plays a decisive role in the tumor response against radiotherapy. Nevertheless, radiation-induced gene alterations on cancer-associated endothelial cells of human endometrial carcinoma remain poorly understood. The purpose of this study was to elucidate the gene expression changes after X-ray radiation in human endometrial carcinoma vascular endothelial cells and to provide new targets for combined treatment of radiation and anti-angiogenesis in human endometrial carcinoma.
Materials And Methods:
Endometrial cancer-derived endothelial cells, which obtained before and 4 h after 400 cGy X-ray radiation from four endometrial carcinomas, were analyzed by gene expression profile. The selected meaningful genes from gene microarray experiments were validated by real-time quantitative PCR.
Results:
Microarray analyses showed 49 significantly changed genes which were common to all the microarray experiments. There into, 14 genes were found to be in persistent up-regulation and 14 in persistent down-regulation 4 h after X-ray radiation when compared with the control group. These genes were involved in cell cycle and growth regulation, cell-apoptosis, chemokine, cell signaling, cellular stress response, angiogenesis, DNA synthesis and repair and cell adhesion. Eight randomly selected genes were validated by real-time PCR.
Discussion:
The genes of cancer-derived endothelial cells regulated by X-ray radiation as well as their related signal pathways, which obtained from gene expression profiling data, were relevant to radiosensitivity of endometrial cancer. This study shows that the identified genes and their related signaling pathways are candidated biomarkers for radiation and anti-angiogenesis of human endometrial carcinoma.
Insights
X-ray radiation alters gene expression in human endometrial carcinoma vascular endothelial cells, impacting tumor radiosensitivity. Identified genes offer potential biomarkers for combined radiation and anti-angiogenesis therapies.
Area of Science:
- Oncology
- Molecular Biology
- Radiotherapy
Background:
- Microvascular endothelial cell death is crucial for radiotherapy response in tumors.
- Radiation-induced gene alterations in cancer-associated endothelial cells of endometrial carcinoma are not well understood.
Purpose of the Study:
- To investigate gene expression changes in human endometrial carcinoma vascular endothelial cells post-X-ray radiation.
- To identify potential targets for combined radiation and anti-angiogenesis therapy in endometrial carcinoma.
Main Methods:
- Gene expression profiling of human endometrial carcinoma-derived endothelial cells before and 4 hours after 400 cGy X-ray radiation.
- Validation of selected genes using real-time quantitative PCR.
Main Results:
- 49 significantly altered genes common across all samples were identified.
- 14 genes showed persistent up-regulation and 14 showed persistent down-regulation 4 hours post-radiation.
- Affected genes are involved in cell cycle, apoptosis, angiogenesis, DNA repair, and cell adhesion.
Conclusions:
- Radiation-regulated genes and pathways in cancer-derived endothelial cells correlate with endometrial cancer radiosensitivity.
- Identified genes and pathways are potential biomarkers for combined radiation and anti-angiogenesis treatment strategies.
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