Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

87.9K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
87.9K
Sanger Sequencing01:57

Sanger Sequencing

800.8K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
800.8K
RNA-seq03:21

RNA-seq

9.4K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.4K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

10.5K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
10.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Draft genomes of 10 skin bacteria isolated from human forearm.

Microbiology resource announcements·2026
Same author

Correction: Maurer et al. Gut Microbial Disruption in Critically Ill Patients with COVID-19-Associated Pulmonary Aspergillosis. <i>J. Fungi</i> 2022, <i>8</i>, 1265.

Journal of fungi (Basel, Switzerland)·2026
Same author

Correction to: Systematic Evaluation of Clinical, Nutritional, and Fecal Microbial Factors for Their Association With Colorectal Polyps.

Clinical and translational gastroenterology·2026
Same author

Abridged Ribosome Profiling for Accurate Bacterial Translation Measurements.

Methods and protocols·2026
Same author

Extrinsic lipids are absorbed and accumulate in colorectal cancer.

Gut·2026
Same author

Bacterial colonized melanoma skin models allow to study host-microbe interactions <i>in situ</i>.

Frontiers in microbiology·2026

Related Experiment Video

Updated: May 4, 2026

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

85.9K

Short barcodes for next generation sequencing.

Katharina Mir1, Klaus Neuhaus2, Martin Bossert1

  • 1Institute of Communications Engineering, Ulm University, Ulm, Germany.

Plos One
|January 4, 2014
PubMed
Summary
This summary is machine-generated.

This study introduces new short DNA barcodes (6-8 nucleotides) for parallel sequencing, focusing on correcting substitution errors. Optimized barcodes are provided, with length-eight codes correcting two errors.

More Related Videos

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
06:24

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

3.4K
An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
10:00

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

18.8K

Related Experiment Videos

Last Updated: May 4, 2026

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

85.9K
Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
06:24

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

3.4K
An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
10:00

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

18.8K

Area of Science:

  • Bioinformatics
  • Computational Biology
  • Genomics

Background:

  • Parallel sequencing platforms are susceptible to substitution errors.
  • Short DNA barcodes are crucial for multiplexing samples in sequencing experiments.
  • Existing barcodes may not meet optimal error correction or biological constraints.

Purpose of the Study:

  • To design and evaluate short DNA barcodes (6-8 nucleotides) for parallel sequencing.
  • To develop new construction methods for error-correcting barcodes.
  • To identify barcodes with superior error correction and biological compatibility.

Main Methods:

  • Comparison of published barcodes with newly constructed codes.
  • Utilizing linear codes and randomized construction methods.
  • Evaluation based on error correction, barcode size, and experimental parameters.

Main Results:

  • New barcode construction methods were developed and evaluated.
  • Codes were generated for lengths 6-8 nucleotides.
  • Length-eight barcodes capable of correcting two substitution errors were identified.

Conclusions:

  • The developed barcodes offer improved error correction for parallel sequencing.
  • The study provides a list of optimized short barcodes for experimental use.
  • The findings contribute to more accurate and efficient high-throughput sequencing.